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公开(公告)号:US09493821B2
公开(公告)日:2016-11-15
申请号:US13989031
申请日:2011-09-21
申请人: Ye Du , Meiru Zhao , Ying Chen , Jinghua Wu , Geng Tian , Jun Wang
发明人: Ye Du , Meiru Zhao , Ying Chen , Jinghua Wu , Geng Tian , Jun Wang
CPC分类号: C12Q1/683 , C12N15/1093 , C12Q1/6869 , C12Q1/6883 , C12Q2521/313 , C12Q2525/191 , C40B50/06
摘要: A DNA library, and a preparing method thereof, a method of determining DNA sequence information, an apparatus and a kit for detecting SNPs, and a method for genotyping may be provided. The method for preparing the DNA library may comprise the steps of: digesting a genomic DNA sample using a restriction endonuclease to obtain a digested product, wherein the restriction endonuclease comprises at least one selected from the group consisting of Mbo II and Tsp 45I; separating the digested product to obtain DNA fragments having a length of 100 bp to 1,000 bp; end-repairing the DNA fragments to obtain an end-repaired DNA fragments; adding a base A to the end of the end-repaired DNA fragments to obtain DNA fragments having a terminal base A; and ligating the DNA fragments having the terminal base A with sequencing adaptors to obtain the DNA library.