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公开(公告)号：US6100091A
公开(公告)日：2000-08-08
申请号：US276295
申请日：1999-03-25
IPC分类号： C12N15/09 , C12N1/15 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/00 , C12N15/11 , C12N15/29 , C12N15/82 , C07H21/04 , C12N5/14
CPC分类号： C12N9/0083 , C12N15/8247 , C07K2319/00
摘要： Disclosed is a methods for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.
摘要翻译： 公开了用于改变可溶性植物脂肪酸去饱和酶的链长和双键位置特异性的方法。 更具体地，该方法包括修饰与脂肪酸接触的可溶性脂肪酸去饱和酶的底物结合通道中的氨基酸接触残基。 具体公开的是酰基-ACP去饱和酶的修饰。 鉴定位于底物结合通道内的氨基酸接触残基，随后用不同的残基替换以影响活性的修饰。
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公开(公告)号：US5705391A
公开(公告)日：1998-01-06
申请号：US689823
申请日：1996-08-14
IPC分类号： C12N15/09 , C12N1/15 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/00 , C12N15/11 , C12N15/29 , C12N15/82 , C12N1/00 , C12N5/00
CPC分类号： C12N9/0083 , C12N15/8247 , C07K2319/00
摘要： Disclosed is a methods for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.
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