公开(公告)号：US10513685B2

公开(公告)日：2019-12-24

申请号：US13636855

申请日：2011-03-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

发明人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

IPC分类号： C12N5/071 ,  C12N5/0735 ,  C12M1/32

摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).

公开(公告)号：US20130071932A1

公开(公告)日：2013-03-21

申请号：US13636855

申请日：2011-03-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

发明人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

IPC分类号： C12N5/071 ,  C12N5/095 ,  C12N5/076 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12M23/12 ,  C12N2502/28 ,  C12N2506/02 ,  C12N2535/10

摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).

摘要翻译： 提供了通过使用其中形成有多个微室的细胞培养室，实现胚状体大小的控制并且可以在控制胚状体大小的状态下诱导分化的方法。 用于引起多能哺乳动物细胞分化的培养方法使用在培养表面上形成有多个微室（11）的细胞培养室（10）。 细胞培养室（10）具有由空间形成的培养面，其中微室（11）具有高度为10μm〜500μm，底面积为100μm2〜1mm2的空间结构。 引起多能哺乳动物细胞分化的培养方法包括通过使用细胞培养室（10）培养多能哺乳动物细胞以获得至少部分分化为内胚层谱系细胞的细胞群。

公开(公告)号：US20140065659A1

公开(公告)日：2014-03-06

申请号：US14005508

申请日：2012-03-16

申请人： Yoko Itchoda ,  Masaya Hosoda ,  Motohiro Fukuda ,  Masafumi Nagayama

发明人： Yoko Itchoda ,  Masaya Hosoda ,  Motohiro Fukuda ,  Masafumi Nagayama

IPC分类号： C12N5/077 ,  G01N33/50

CPC分类号： C12N5/0653 ,  C12M23/12 ,  C12M25/06 ,  C12N2513/00 ,  G01N33/5044

摘要： In a culture method for adipocytes, seeded adipocytes are cultured while being allowed to adhere to a culture bottom surface (14) and walls (12) disposed perpendicularly to the culture bottom surface (14), without suspending the adipocytes in a culture solution, thereby obtaining mature adipocytes in which spherical lipid droplets having increased in size are generated within cells. This method uses a culture chamber (10) in which the walls (12) are formed perpendicular to the culture bottom surface (14). Spherical mature adipocytes containing spherical lipid droplets having increased in size within cells are cultured in a state where a cell or an aggregate of cells is allowed to adhere to two or more locations on the walls (12) and the culture bottom surface (14), thereby obtaining adipocytes having a shape similar to that of adipocytes in vivo.

摘要翻译： 在脂肪细胞的培养方法中，将接种的脂肪细胞培养同时粘附到培养基底表面（14）和垂直于培养物底表面（14）设置的壁（12），而不将脂肪细胞悬浮在培养溶液中，从而 获得其中在细胞内产生尺寸增加的球形脂滴的成熟脂肪细胞。 该方法使用其中壁（12）垂直于培养物底表面（14）形成的培养室（10）。 在允许细胞或细胞聚集体粘附在壁（12）和培养底表面（14）上的两个或更多位置的状态下培养含有细胞内尺寸增加的球形脂滴的球形成熟脂肪细胞， 从而获得具有与体内脂肪细胞相似形状的脂肪细胞。

公开(公告)号：US20110207215A1

公开(公告)日：2011-08-25

申请号：US13125871

申请日：2009-10-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

发明人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

IPC分类号： C12N5/071 ,  C12N5/0735 ,  C12N5/0793 ,  C12N5/077

CPC分类号： A01N1/0263 ,  C12M23/12 ,  C12M23/24 ,  C12M23/38 ,  C12M23/54 ,  C12M45/22

摘要： To provide a cell storage method to store living cells while maintaining the functions of the living cells. An aspect of the cell storage method is a method to store living cells (40) by using a cell culture chamber (20) including a plurality of the microchambers (11), the method including: culturing the living cells by being adhered to the surfaces of a plurality of micro spaces; and after the culturing, pouring the culture medium (50) into the cell culture chamber (20) so as to cover the plurality of microchambers (11), and storing the living cells. The living cells are adhered to a cell culture chamber of an appropriate size and cultured to form a three-dimensional structure. This enables maintaining the three-dimensional structure of the living cells and storage of the living cells while maintaining the functions thereof.

摘要翻译： 提供细胞储存方法来储存活细胞同时保持活细胞的功能。 细胞储存方法的一个方面是通过使用包括多个微室（11）的细胞培养室（20）来储存活细胞（40）的方法，所述方法包括：通过粘附到所述表面上培养活细胞 的多个微空间; 培养后，将培养基（50）注入到细胞培养室（20）中以覆盖多个微室（11），并储存活细胞。 将活细胞粘附到适当尺寸的细胞培养室并进行培养以形成三维结构。 这能够维持活细胞的三维结构并维持活体的功能。

公开(公告)号：US20110217725A1

公开(公告)日：2011-09-08

申请号：US13125896

申请日：2009-10-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

发明人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

IPC分类号： C12N5/07 ,  C12Q1/02

CPC分类号： C12N5/0062 ,  C12M23/12 ,  C12N2502/00 ,  C12N2503/02 ,  C12N2513/00

摘要： To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (33) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (33). Specifically, living cells D1, D2, and D3 derived from various donors are adhered to the plurality of microchambers (33). In each microchamber (33), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.

摘要翻译： 提供包括各种供体的培养的活细胞的细胞培养试剂盒及其制造方法。 细胞培养试剂盒包括培养板和在其上培养的活细胞。 培养板包括多个微室（33），并且来自各种供体的活细胞粘附到多个微室（33）的表面。 具体而言，将来自各种供体的活细胞D1，D2和D3粘附到多个微室（33）。 在每个微室（33）中，可以培养衍生自一个供体的活细胞或来自各种供体的活细胞。 来自各种供体的活细胞作为整体在细胞培养试剂盒中附着和培养，这使得可以提供使用来自各种供体的细胞进行测试的细胞培养试剂盒。