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14 条记录 (耗时 0.025 秒)

    CELL CULTURE METHOD AND SCREENING METHOD
    1.
    发明申请
    CELL CULTURE METHOD AND SCREENING METHOD 有权
    细胞培养方法和筛选方法

    公开(公告)号：US20110045500A1

    公开(公告)日：2011-02-24

    申请号：US12866581

    申请日：2009-02-05

    申请人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

    发明人： Hideki Taniguchi ,  Yun-Wen Zheng ,  Go Tazaki ,  Tomoko Kosaka ,  Hitoshi Tsuruta ,  Motohiro Fukuda

    IPC分类号： G01N33/53 ,  C12N5/071 ,  C12N5/079

    CPC分类号： C12M23/22 ,  C12M23/12 ,  G01N33/5073

    摘要： Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.

    摘要翻译： 本发明提供能够长期维持体内功能的细胞培养方法，并且可以使用所需的最少细胞数进行培养。 细胞培养方法包括在分层微空间中以分层状态培养未分化细胞，获得分化细胞。 当筛选药剂时，优选能够分化为肝细胞，肠上皮细胞，神经细胞，心肌细胞和血管内皮细胞的未分化细胞。 特别是在人体的药代动力学等的预测中，优选人细胞。

    CELL CULTURE METHOD
    2.
    发明申请
    CELL CULTURE METHOD 有权
    细胞培养方法

    公开(公告)号：US20100331216A1

    公开(公告)日：2010-12-30

    申请号：US12866588

    申请日：2009-02-05

    申请人： Masahiro Sokabe ,  Kishio Furuya ,  Go Tazaki ,  Hitoshi Tsuruta ,  Motohiro Fukuda

    发明人： Masahiro Sokabe ,  Kishio Furuya ,  Go Tazaki ,  Hitoshi Tsuruta ,  Motohiro Fukuda

    IPC分类号： C40B50/06

    CPC分类号： C12N5/0631 ,  C12M23/12 ,  C12M23/16 ,  C12M23/22 ,  C12N5/0062 ,  C12N2535/10

    摘要： To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.

    摘要翻译： 提供能够通过抑制去分化来长时间维持体内功能的细胞培养方法。 根据本发明的细胞培养方法是在微小分隔空间中以多层状态培养细胞，并获得具有类似于体内功能的功能的组织结构。 当细胞是乳腺上皮细胞时，可以形成中空的类固醇样结构。 微细分隔空间特别优选为细胞培养容器中的微型容器，其具有表面上的多个这种微量容器。

    Apparatus for the determination of a trace amount of an analyte substance or organism
    5.
    发明授权
    Apparatus for the determination of a trace amount of an analyte substance or organism 失效
    一种用于分析物物质或生物体的痕迹量的测定

    公开(公告)号：US5674696A

    公开(公告)日：1997-10-07

    申请号：US328057

    申请日：1994-10-24

    申请人： Michihiro Nakamura ,  Satomi Matsui ,  Keiko Oka ,  Hitoshi Tsuruta

    发明人： Michihiro Nakamura ,  Satomi Matsui ,  Keiko Oka ,  Hitoshi Tsuruta

    IPC分类号： G01N33/53 ,  G01N27/416 ,  G01N33/543 ,  G01N27/327 ,  G01N33/569

    CPC分类号： G01N27/4145 ,  Y10S435/817 ,  Y10S436/806

    摘要： There is disclosed an apparatus for the determination of a trace amount of an analyte substance or organism. A substrate solution is brought into contact with a small diameter tube which has captured, at least on its inner surface, a trace amount of an analyte substance or organism. The trace amount has the ability to change the pH of the substrate solution. A pH change following a reaction of a substrate in the substrate solution in the small diameter tube is measured; the apparatus has a cuvette having an inlet and an outlet for the substrate solution, a pH electrode accomodated in the cuvette, a pump for supplying the substrate solution in the cuvette, a positioning structure for positioning, in the cuvette, the small diameter tube with the pH sensitive plane of the pH electrode and spacing the surface and the plane at 1 mm or below from each other, and a sealing structure for maintaining a formed by the outer surface of the small diameter tube accommodated in the cuvette and the inner surface of the cuvette at liquid tight condition.

    摘要翻译： 公开了一种用于分析物物质或生物体的痕迹量的测定的装置。 将底物溶液与小直径管接触，其至少在其内表面捕获痕量的被分析物质或生物体。 痕量具有改变的底物溶液的pH的能力。 测量小直径管中底物溶液中底物反应后的pH变化; 该装置具有一个具有用于底物溶液的入口和出口的比色杯，一个容纳在该比色皿中的pH电极，一个用于将该基质溶液供应到该比色杯中的泵，一个定位结构，用于在该小杯中定位该小直径管， pH电极的pH敏感平面，并将表面和平面彼此间隔1mm或1mm以下，并且保持由容纳在反应杯中的小直径管的外表面形成的密封结构， 液体条件下的比色杯。

    CELL STORAGE METHOD AND CELL TRANSPORT METHOD
    6.
    发明申请
    CELL STORAGE METHOD AND CELL TRANSPORT METHOD 审中-公开
    细胞储存方法和细胞运输方法

    公开(公告)号：US20110207215A1

    公开(公告)日：2011-08-25

    申请号：US13125871

    申请日：2009-10-23

    申请人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

    发明人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

    IPC分类号： C12N5/071 ,  C12N5/0735 ,  C12N5/0793 ,  C12N5/077

    CPC分类号： A01N1/0263 ,  C12M23/12 ,  C12M23/24 ,  C12M23/38 ,  C12M23/54 ,  C12M45/22

    摘要： To provide a cell storage method to store living cells while maintaining the functions of the living cells. An aspect of the cell storage method is a method to store living cells (40) by using a cell culture chamber (20) including a plurality of the microchambers (11), the method including: culturing the living cells by being adhered to the surfaces of a plurality of micro spaces; and after the culturing, pouring the culture medium (50) into the cell culture chamber (20) so as to cover the plurality of microchambers (11), and storing the living cells. The living cells are adhered to a cell culture chamber of an appropriate size and cultured to form a three-dimensional structure. This enables maintaining the three-dimensional structure of the living cells and storage of the living cells while maintaining the functions thereof.

    摘要翻译： 提供细胞储存方法来储存活细胞同时保持活细胞的功能。 细胞储存方法的一个方面是通过使用包括多个微室（11）的细胞培养室（20）来储存活细胞（40）的方法，所述方法包括：通过粘附到所述表面上培养活细胞 的多个微空间; 培养后，将培养基（50）注入到细胞培养室（20）中以覆盖多个微室（11），并储存活细胞。 将活细胞粘附到适当尺寸的细胞培养室并进行培养以形成三维结构。 这能够维持活细胞的三维结构并维持活体的功能。

    CELL CULTURE KIT, SCREENING METHOD, AND METHOD OF MANUFACTURING CELL CULTURE KIT
    8.
    发明申请
    CELL CULTURE KIT, SCREENING METHOD, AND METHOD OF MANUFACTURING CELL CULTURE KIT 审中-公开
    细胞培养试剂盒，筛选方法和制备细胞培养试剂盒的方法

    公开(公告)号：US20110217725A1

    公开(公告)日：2011-09-08

    申请号：US13125896

    申请日：2009-10-23

    申请人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

    发明人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

    IPC分类号： C12N5/07 ,  C12Q1/02

    CPC分类号： C12N5/0062 ,  C12M23/12 ,  C12N2502/00 ,  C12N2503/02 ,  C12N2513/00

    摘要： To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (33) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (33). Specifically, living cells D1, D2, and D3 derived from various donors are adhered to the plurality of microchambers (33). In each microchamber (33), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.

    摘要翻译： 提供包括各种供体的培养的活细胞的细胞培养试剂盒及其制造方法。 细胞培养试剂盒包括培养板和在其上培养的活细胞。 培养板包括多个微室（33），并且来自各种供体的活细胞粘附到多个微室（33）的表面。 具体而言，将来自各种供体的活细胞D1，D2和D3粘附到多个微室（33）。 在每个微室（33）中，可以培养衍生自一个供体的活细胞或来自各种供体的活细胞。 来自各种供体的活细胞作为整体在细胞培养试剂盒中附着和培养，这使得可以提供使用来自各种供体的细胞进行测试的细胞培养试剂盒。