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公开(公告)号：US10513685B2
公开(公告)日：2019-12-24
申请号：US13636855
申请日：2011-03-23
申请人： Yoko Itchoda , Go Tazaki , Masaya Hosoda , Motohiro Fukuda , Hideki Taniguchi , Yun-Wen Zheng , Keisuke Sekine
发明人： Yoko Itchoda , Go Tazaki , Masaya Hosoda , Motohiro Fukuda , Hideki Taniguchi , Yun-Wen Zheng , Keisuke Sekine
IPC分类号： C12N5/071 , C12N5/0735 , C12M1/32
摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).
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公开(公告)号：US08435782B2
公开(公告)日：2013-05-07
申请号：US12280607
申请日：2007-02-19
申请人： Taiji Nishi , Go Tazaki , Motohiro Fukuda , Michio Yazawa , Masayuki Takahashi
发明人： Taiji Nishi , Go Tazaki , Motohiro Fukuda , Michio Yazawa , Masayuki Takahashi
IPC分类号： C12M1/14
CPC分类号： C12M23/12 , B01L3/5085 , B01L3/5088 , B01L2300/0829 , B01L2300/0851 , B01L2300/0858 , B01L2300/0893 , C12M25/00
摘要： An object of the present invention is to provide a cell culture chamber with which the survival rate of cells to be cultured can be increased, and a method of producing the same. A cell culture chamber according to the present invention includes a mass of projections formed of a plurality of microprojections 1 formed on a surface on which cells are cultured. The width or diameter of each of the microprojections 1 is in a range of 20 nm to 3 μm, and the aspect ratio of each of the microprojections is in a range of 0.2 to 3.0. Thus, there can be provided a cell culture chamber most suitable for the adhesion and differentiation/proliferation of cells to be cultured.
摘要翻译： 本发明的目的是提供一种能够提高培养细胞存活率的细胞培养室及其制造方法。 根据本发明的细胞培养室包括由在其上培养细胞的表面上形成的多个微突出物1形成的突起体。 每个微喷射体1的宽度或直径在20nm至3μm的范围内，并且每个微喷射体的纵横比在0.2至3.0的范围内。 因此，可以提供最适合于待培养细胞的粘附和分化/增殖的细胞培养室。
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公开(公告)号：US08415157B2
公开(公告)日：2013-04-09
申请号：US13229087
申请日：2011-09-09
申请人： Go Tazaki , Tomoko Kosaka , Motohiro Fukuda
发明人： Go Tazaki , Tomoko Kosaka , Motohiro Fukuda
摘要： A cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space are provided. A cell culture chamber (10)according to the present invention is a cell culture chamber (10)including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.
摘要翻译： 提供了能够有效地构建培养空间中的细胞间网络的细胞培养室和细胞培养方法。 根据本发明的细胞培养室（10）是包括形成在其表面上的多个微室（11）的细胞培养室（10），其特征在于，将微型室（11）分隔开的凸部（侧壁12） ）形成为防止细胞粘附到凸部的上表面。 因此，可以在微室（11）内专门培养细胞，并且可以有效地构建细胞间网络。
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公开(公告)号：US20110045500A1
公开(公告)日：2011-02-24
申请号：US12866581
申请日：2009-02-05
申请人： Hideki Taniguchi , Yun-Wen Zheng , Go Tazaki , Tomoko Kosaka , Hitoshi Tsuruta , Motohiro Fukuda
发明人： Hideki Taniguchi , Yun-Wen Zheng , Go Tazaki , Tomoko Kosaka , Hitoshi Tsuruta , Motohiro Fukuda
CPC分类号： C12M23/22 , C12M23/12 , G01N33/5073
摘要： Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.
摘要翻译： 本发明提供能够长期维持体内功能的细胞培养方法，并且可以使用所需的最少细胞数进行培养。 细胞培养方法包括在分层微空间中以分层状态培养未分化细胞，获得分化细胞。 当筛选药剂时，优选能够分化为肝细胞，肠上皮细胞，神经细胞，心肌细胞和血管内皮细胞的未分化细胞。 特别是在人体的药代动力学等的预测中，优选人细胞。
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公开(公告)号：US20100331216A1
公开(公告)日：2010-12-30
申请号：US12866588
申请日：2009-02-05
申请人： Masahiro Sokabe , Kishio Furuya , Go Tazaki , Hitoshi Tsuruta , Motohiro Fukuda
发明人： Masahiro Sokabe , Kishio Furuya , Go Tazaki , Hitoshi Tsuruta , Motohiro Fukuda
IPC分类号： C40B50/06
CPC分类号： C12N5/0631 , C12M23/12 , C12M23/16 , C12M23/22 , C12N5/0062 , C12N2535/10
摘要： To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.
摘要翻译： 提供能够通过抑制去分化来长时间维持体内功能的细胞培养方法。 根据本发明的细胞培养方法是在微小分隔空间中以多层状态培养细胞，并获得具有类似于体内功能的功能的组织结构。 当细胞是乳腺上皮细胞时，可以形成中空的类固醇样结构。 微细分隔空间特别优选为细胞培养容器中的微型容器，其具有表面上的多个这种微量容器。
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公开(公告)号：US07569638B2
公开(公告)日：2009-08-04
申请号：US11587921
申请日：2005-04-25
申请人： Hiroshi Ozawa , Yuichi Katoh , Motohiro Fukuda
发明人： Hiroshi Ozawa , Yuichi Katoh , Motohiro Fukuda
IPC分类号： C08L51/04
CPC分类号： C08L51/06 , C08F255/02 , C08F255/026 , C08L23/0853 , C08L2666/24 , C08L2666/04 , C08L2666/02
摘要： To provide a thermoplastic elastomer composition which is excellent in light resistance and flexibility and has high tear strength. [MEANS FOR SOLVING PROBLEMS] A thermoplastic elastomer composition comprising 100 parts by mass of acrylic multi-layer polymer particles (A) which each comprise at least two layers, i.e., at least one inner rubber layer (I) and at least one outermost thermoplastic resin layer (II), 5 to 300 parts by mass of an ethylene/vinyl ester copolymer (B), and 0.5 to 150 parts by mass of a graft copolymer (C) composed of an ethylene/vinyl ester copolymer backbone and a graft bonded to the backbone which consists of units derived from a monomer having a polarity higher than that of the backbone.
摘要翻译： 提供耐光性和柔软性优异且撕裂强度高的热塑性弹性体组合物。 [解决问题的手段]一种热塑性弹性体组合物，其包含100质量份丙烯酸多层聚合物颗粒（A），其各自包含至少两层，即至少一层内橡胶层（I）和至少一种最外层热塑性塑料 树脂层（II），乙烯/乙烯基酯共聚物（B）5〜300质量份，乙烯/乙烯基酯共聚物主链和接枝共聚物主链组成的接枝共聚物（C）0.5〜150质量份 到由具有高于骨架的极性的单体衍生的单元组成的骨架。
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公开(公告)号：US09005970B2
公开(公告)日：2015-04-14
申请号：US11813720
申请日：2006-01-11
申请人： Ryosuke Nishio , Taiji Nishi , Motohiro Fukuda , Yasuzo Kirita , Seiichi Kanai , Takenori Kitani
发明人： Ryosuke Nishio , Taiji Nishi , Motohiro Fukuda , Yasuzo Kirita , Seiichi Kanai , Takenori Kitani
CPC分类号： C12M25/06 , C12M23/12 , C12N5/0657 , C12N2533/30 , C12N2535/00
摘要： [PROBLEMS] Relating to a bioassay method with the use of cultured cells and cell culture for a therapeutic purpose in the case of determining the efficacy of a drug or the like or examining the toxicity thereof, it is intended to provide a method of culturing cells under regulation in the extension direction and a cell culture plate. [MEANS FOR SOLVING PROBLEMS] A cell culture plate having a plural number of side walls (1) and a plural number of space structures (3) for providing cultured cells which are formed by the side walls (1), wherein the side walls (1) are provided with openings (2) so that the space structures (3) are linked together. By using this cell culture plate, a method of culturing cells under regulation in the extension direction can be provided.
摘要翻译： [问题]关于在确定药物等的功效或检查其毒性的情况下使用培养细胞和细胞培养物进行生物测定方法的目的是提供培养细胞的方法 在延伸方向调节和细胞培养板。 解决问题的手段具有多个侧壁（1）和多个用于提供由侧壁（1）形成的培养细胞的多个空间结构（3）的细胞培养板，其中侧壁 1）设置有开口（2），使得空间结构（3）连接在一起。 通过使用该细胞培养板，可以提供在延伸方向的调节下培养细胞的方法。
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公开(公告)号：US08980625B2
公开(公告)日：2015-03-17
申请号：US11360535
申请日：2006-02-24
申请人： Yuji Kikuchi , Hiroko Kikuchi , Taiji Nishi , Takenori Kitani , Yukihiro Yanagawa , Motohiro Fukuda
发明人： Yuji Kikuchi , Hiroko Kikuchi , Taiji Nishi , Takenori Kitani , Yukihiro Yanagawa , Motohiro Fukuda
CPC分类号： C12M23/16 , B01L3/5027
摘要： A cell culture plate has a plurality of flow channels, uneven pattern areas and through holes. The flow channels are formed between the uneven pattern areas, and culture solution flows from the flow channels into the uneven pattern areas or from the uneven pattern areas into the flow channels. The uneven pattern area has uneven patterns that create a cell culture space.
摘要翻译： 细胞培养板具有多个流路，不均匀图案区域和通孔。 流动通道形成在不均匀图案区域之间，培养液从流动通道流入不均匀图案区域或从凹凸图案区域流入流动通道。 不均匀图案区域具有形成细胞培养空间的不均匀图案。
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公开(公告)号：US20090170190A1
公开(公告)日：2009-07-02
申请号：US12280607
申请日：2007-02-19
申请人： Taiji Nishi , Go Tazaki , Motohiro Fukuda , Michio Yazawa , Masayuki Takahashi
发明人： Taiji Nishi , Go Tazaki , Motohiro Fukuda , Michio Yazawa , Masayuki Takahashi
CPC分类号： C12M23/12 , B01L3/5085 , B01L3/5088 , B01L2300/0829 , B01L2300/0851 , B01L2300/0858 , B01L2300/0893 , C12M25/00
摘要： An object of the present invention is to provide a cell culture chamber with which the survival rate of cells to be cultured can be increased, and a method of producing the same. A cell culture chamber according to the present invention includes a mass of projections formed of a plurality of microprojections 1 formed on a surface on which cells are cultured. The width or diameter of each of the microprojections 1 is in a range of 20 nm to 3 μm, and the aspect ratio of each of the microprojections is in a range of 0.2 to 3.0. Thus, there can be provided a cell culture chamber most suitable for the adhesion and differentiation/proliferation of cells to be cultured.
摘要翻译： 本发明的目的是提供一种能够提高培养细胞存活率的细胞培养室及其制造方法。 根据本发明的细胞培养室包括由在其上培养细胞的表面上形成的多个微突出物1形成的突起体。 每个微喷射体1的宽度或直径在20nm至3μm的范围内，并且每个微喷射体的纵横比在0.2至3.0的范围内。 因此，可以提供最适合于待培养细胞的粘附和分化/增殖的细胞培养室。
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公开(公告)号：US20090075363A1
公开(公告)日：2009-03-19
申请号：US12298803
申请日：2007-04-25
申请人： Yuji Morimoto , Taiji Nishi , Taisuke Kamada , Go Tazaki , Motohiro Fukuda
发明人： Yuji Morimoto , Taiji Nishi , Taisuke Kamada , Go Tazaki , Motohiro Fukuda
摘要： To provide a cell culture chamber capable of reproducing a cell function in vivo, and a method of producing the same. The cell culture chamber according to the present invention has a concave-convex pattern formed on the surface on which cells are cultured. A concave portion of the concave-convex pattern includes cell culture portions and micro flow channels communicating with the cell culture portions. The bottom surface of each of the cell culture portions has a width 1.0 to 20 times an equivalent diameter of each of the cultured cells. A cell adhesion-inducing substance is formed only on the bottom surface and the side walls of the concave portion.
摘要翻译： 提供能够在体内再现细胞功能的细胞培养室及其制造方法。 根据本发明的细胞培养室具有形成在其上培养细胞的表面上的凹凸图案。 凹凸图案的凹部包括与细胞培养部连通的细胞培养部和微流路。 每个细胞培养部分的底表面具有每个培养细胞的当量直径的1.0至20倍的宽度。 细胞粘附诱导物质仅形成在凹部的底面和侧壁上。
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