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    High throughput system for producing recombinant viruses using site-specific recombination
    1.
    发明授权
    High throughput system for producing recombinant viruses using site-specific recombination 有权
    用于使用位点特异性重组产生重组病毒的高通量系统

    公开(公告)号：US07319001B2

    公开(公告)日：2008-01-15

    申请号：US10383838

    申请日：2003-03-07

    申请人： Ho-Sun Son ,  Do-Hui Kim ,  Neon-C Jung ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Min-Sung Kim ,  Yong-Weon Yi ,  Kyung-Jin Kim

    发明人： Ho-Sun Son ,  Do-Hui Kim ,  Neon-C Jung ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Min-Sung Kim ,  Yong-Weon Yi ,  Kyung-Jin Kim

    IPC分类号： C12N15/10 ,  C12N15/63 ,  C12N15/64 ,  C12N15/866 ,  C12N15/86 ,  A61K48/00

    CPC分类号： C12N15/86 ,  C12N15/1027 ,  C12N2710/14143

    摘要： Disclosed are the methods for producing recombinant viruses using site-specific recombination in vitro. In the present invention, circular viral genomic DNAs are digested with restriction enzymes to generate a linear form viral genomic DNAs flanked by site-specific recombination sites, and then are subjected to site-specific recombination with the desired genomic materials flanked by site-specific recombination sites in vitro. According to the present invention, since the site-specific recombination mixture can be applied to host cells without further procedures of selecting the desired recombinant viral genomic DNAs, it is possible to obtain numerous recombinant viruses rapidly at the same time. Thus, the present invention can be used as a high throughput system for generating and screening hundreds or thousands of recombinant viruses.

    摘要翻译： 公开了使用体外位点特异性重组生产重组病毒的方法。 在本发明中，循环病毒基因组DNA用限制性酶消化以产生侧翼为位点特异性重组位点的线性形式的病毒基因组DNA，然后进行与所需基因组物质的位点特异性重组，所述基因组物质侧接于位点特异性重组 体外试验。 根据本发明，由于可以不用进一步选择所需的重组病毒基因组DNA的方式将位点特异性重组混合物施用于宿主细胞，所以可以同时快速获得多种重组病毒。 因此，本发明可以用作产生和筛选数百或数千种重组病毒的高通量系统。