公开(公告)号：US20160010099A1

公开(公告)日：2016-01-14

申请号：US14771483

申请日：2014-02-28

申请人： Young Woo JIN ,  Eun Kyong LEE ,  UNHWA CORPORATION

发明人： Young Woo JIN ,  Eun Kyong LEE ,  Mi Ok JANG ,  Bora PARK ,  Soo Ran LEE ,  Bo Rim YANG ,  Il Suk KIM ,  Il Seok OH

IPC分类号： C12N15/82

CPC分类号： C12N15/8205 ,  C12N5/04 ,  C12N15/8257

摘要： Plant cells express a target protein, a production method thereof, and a method of producing a target protein using the same are disclosed. The plant cells have introduced therein a recombinant vector containing gene(s) encoding a target protein, and the plant cells include plant cambial meristematic cells (CMC) or callus. The cambial meristematic cells (CMCs) are a cell line containing innately undifferentiated cells isolated from a plant, wherein the cell line is a cell line isolated from the cambial tissue of the plant and has meristematic continuity without going through dedifferentiation into callus. A system of expressing a target protein using the recombinant plant cells according to the present invention can overcome the problems of conventional plant cell culture. In addition, it shows significantly high transformation efficiency, and thus can produce large amounts of target proteins, including biopharmaceutical proteins.

摘要翻译： 公开了植物细胞表达靶蛋白，其生产方法和使用其的生产靶蛋白的方法。 植物细胞在其中引入含有编码靶蛋白的基因的重组载体，植物细胞包括植物成分分生细胞（CMC）或愈伤组织。 成像分生细胞（CMC）是含有从植物分离的天然未分化细胞的细胞系，其中细胞系是从植物的成像组织分离的细胞系，并且具有分生组织连续性，而不经过去分化成愈伤组织。 使用根据本发明的重组植物细胞表达靶蛋白的系统可以克服常规植物细胞培养的问题。 此外，它显示出显着的转化效率，因此可产生大量靶蛋白，包括生物制药蛋白。

公开(公告)号：US20110033903A1

公开(公告)日：2011-02-10

申请号：US12889026

申请日：2010-09-23

申请人： Young Woo Jin

发明人： Young Woo Jin

IPC分类号： C12P17/02 ,  C12N5/04 ,  C12P1/00

CPC分类号： C12N5/04

摘要： This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from cambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture.

摘要翻译： 本发明是通过均质细胞系发育来最小化细胞生长和产生变化的方法。 更具体地说，它是从形成层分离和增殖单细胞克隆的方法，通过解决长期培养中细胞生长减少和生产力的问题，促进植物来源的生物活性物质生产的稳定性。

公开(公告)号：US09314492B2

公开(公告)日：2016-04-19

申请号：US13127750

申请日：2009-11-06

申请人： Young Woo Jin ,  Eun Kyong Lee

发明人： Young Woo Jin ,  Eun Kyong Lee

IPC分类号： A01N65/00 ,  A61K36/258 ,  A01H4/00 ,  A23L1/30 ,  C12N5/04

CPC分类号： A61K36/258 ,  A01H4/005 ,  A23L33/105 ,  A23V2002/00 ,  C12N5/04 ,  A23V2200/308 ,  A23V2250/21

摘要： The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Panax ginseng cambium-derived cell line including wild ginseng or ginseng; a lysate thereof; an extract thereof; or a culture medium thereof, which are derived from a natural and have minimized side effects compared to the conventional therapeutic drugs, and thus are safe for the human body. Also, they are involved directly in the growth of cancer to induce cancer cell death effectively, and show anticancer activity of inhibiting or reducing the formation of tumor or the growth of tumor.

摘要翻译： 本发明涉及一种预防或治疗癌症的组合物，其含有人参或人参的人参形成层细胞系作为活性成分， 其裂解物; 其提取物; 或其培养基，其与常规治疗药物相比源自天然并且具有最小化的副作用，因此对于人体是安全的。 而且，它们直接参与癌细胞的生长，有效地诱导癌细胞死亡，并且显示抑制或减少肿瘤形成或肿瘤生长的抗癌活性。

公开(公告)号：US08617621B2

公开(公告)日：2013-12-31

申请号：US13202868

申请日：2010-02-23

申请人： Min Jung Lim ,  Young Woo Jin ,  Eun Kyong Lee

发明人： Min Jung Lim ,  Young Woo Jin ,  Eun Kyong Lee

IPC分类号： A61K36/00 ,  A61K36/25 ,  A61K39/00 ,  A61K45/00 ,  C12N5/00

CPC分类号： A61K36/258 ,  A01H4/005 ,  A23L33/105 ,  A23V2002/00 ,  C12N5/04 ,  A23V2200/324 ,  A23V2250/2124

摘要： The present invention relates to a composition for enhancing immunity, comprising one or more of the following: a homogenous cell line, and a lysate, an extract and a culture medium thereof as an active ingredient. The homogenous cell line, the lysate, the extract and the culture thereof according to the present invention, which are derived from a natural product, minimize adverse side effects of prior immune enhancing agents and safe for the human body. Further, they effectively increase the activity of NK cells responsible for innate immunity, as well as increase the proliferation rate of lymph node cells when the cells were re-exposed to an antigen in a specific immune reaction to enhance acquired immunity, and thus are useful as an immune enhancing agent. In particular, the homogenous cell line, the lysate, the extract, and the culture thereof according to the present invention also effectively increase the number of hone marrow cells, thus are not only used as an adjuvant in an immune reaction, but also used in the prevention and treatment of anemia through hematopoiesis.

摘要翻译： 本发明涉及一种用于增强免疫力的组合物，其包含以下一种或多种：均质细胞系，其裂解物，提取物和培养基作为活性成分。 衍生自天然产物的根据本发明的均质细胞系，裂解物，提取物及其培养物使先前的免疫增强剂的不良副作用最小化并且对人体而言是安全的。 此外，它们有效地增加负责先天免疫的NK细胞的活性，并且当细胞在特异性免疫反应中再次暴露于抗原以增强获得性免疫时增加淋巴结细胞的增殖率，因此是有用的 作为免疫增强剂。 特别是根据本发明的均质细胞系，裂解物，提取物及其培养物也有效地增加了骨髓细胞的数量，因此不仅用作免疫反应中的佐剂，而且还用于 通过造血预防和治疗贫血。

公开(公告)号：US20120039918A1

公开(公告)日：2012-02-16

申请号：US13202868

申请日：2010-02-23

申请人： Min Jung Lim ,  Young Woo Jin ,  Eun Kyong Lee

发明人： Min Jung Lim ,  Young Woo Jin ,  Eun Kyong Lee

IPC分类号： A61K36/258 ,  A61P37/04 ,  A61P7/06 ,  C12N5/04

CPC分类号： A61K36/258 ,  A01H4/005 ,  A23L33/105 ,  A23V2002/00 ,  C12N5/04 ,  A23V2200/324 ,  A23V2250/2124

摘要： The present invention relates to a composition for enhancing immunity, comprising one or more of the following: a homogenous cell line, and a lysate, an extract and a culture medium thereof as an active ingredient. The homogenous cell line, the lysate, the extract and the culture thereof according to the present invention, which are derived from a natural product, minimize adverse side effects of prior immune enhancing agents and safe for the human body. Further, they effectively increase the activity of NK cells responsible for innate immunity, as well as increase the proliferation rate of lymph node cells when the cells were re-exposed to an antigen in a specific immune reaction to enhance acquired immunity, and thus are useful as an immune enhancing agent. In particular, the homogenous cell line, the lysate, the extract, and the culture thereof according to the present invention also effectively increase the number of hone marrow cells, thus are not only used as an adjuvant in an immune reaction, but also used in the prevention and treatment of anemia through hematopoiesis.

摘要翻译： 本发明涉及一种用于增强免疫力的组合物，其包含以下一种或多种：均质细胞系，其裂解物，提取物和培养基作为活性成分。 衍生自天然产物的根据本发明的均质细胞系，裂解物，提取物及其培养物使先前的免疫增强剂的不良副作用最小化并且对人体而言是安全的。 此外，它们有效地增加负责先天免疫的NK细胞的活性，并且当细胞在特异性免疫反应中再次暴露于抗原以增强获得性免疫时增加淋巴结细胞的增殖速率，因此是有用的 作为免疫增强剂。 特别是根据本发明的均质细胞系，裂解物，提取物及其培养物也有效地增加了骨髓细胞的数量，因此不仅用作免疫反应中的佐剂，而且还用于 通过造血预防和治疗贫血。

公开(公告)号：US20110039312A1

公开(公告)日：2011-02-17

申请号：US12889126

申请日：2010-09-23

申请人： Young Woo Jin

发明人： Young Woo Jin

IPC分类号： C12P17/02 ,  C12N5/04

CPC分类号： C12N5/04

摘要： This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from procambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture.

摘要翻译： 本发明是通过均质细胞系发育来最小化细胞生长和产生变化的方法。 更具体地说，它是通过解决细胞生长减少和长期培养中的生产力的问题，从原核中分离和增殖单细胞克隆的方法来促进植物来源的生物活性物质生产的稳定性。

公开(公告)号：US20100233813A1

公开(公告)日：2010-09-16

申请号：US12679269

申请日：2008-09-22

申请人： Mi Ok Jang ,  Eun Kyong Lee ,  Young Woo Jin

发明人： Mi Ok Jang ,  Eun Kyong Lee ,  Young Woo Jin

IPC分类号： C12N5/04

CPC分类号： C12N5/04

摘要： The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time. Accordingly, the cell line derived from the cambium of an herbaceous plant having a storage root makes it possible to produce large amounts of useful plants which are difficult to cultivate outdoor due to various problems associated with the period of cultivation, the selection of cultivation land, cultivation cost and the like.

摘要翻译： 本发明涉及源自具有贮藏根的草本植物的形成层的细胞系及其分离方法。 更具体地说，涉及具有分割能力的形成层的均质细胞系，其是从没有分离去分化方法的具有储存根的草本植物的含有形成层的储存组织获得的，以及其分离方法 。 源自具有储存根的草本植物的形成层的细胞系具有活性分裂能力并且是均匀的。 此外，它在培养期间是稳定的，因为它没有经历去分化过程。 因此，通过优化其增殖，可以在短时间内允许细胞系大量增殖。 因此，从具有储藏根的草本植物的形成层衍生的细胞系，由于与栽培期间，种植面积的选择有关的各种问题，可能产生大量难以栽培的有用植物， 栽培成本等。

公开(公告)号：US20130137178A1

公开(公告)日：2013-05-30

申请号：US13551207

申请日：2012-07-17

申请人： Mi Ok Jang ,  Eun Kyong Lee ,  Young Woo Jin

发明人： Mi Ok Jang ,  Eun Kyong Lee ,  Young Woo Jin

IPC分类号： C12N5/04

CPC分类号： C12N5/04

摘要： The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has an active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time.

摘要翻译： 本发明涉及源自具有贮藏根的草本植物的形成层的细胞系及其分离方法。 更具体地说，涉及具有分割能力的形成层的均质细胞系，其具有不具有分离去分化方法的具有储存根的草本植物的含有形成层的存储组织，以及其分离方法 。 源自具有储存根的草本植物的形成层的细胞系具有活性分裂能力并且是均匀的。 此外，它在培养期间是稳定的，因为它没有经历去分化过程。 因此，通过优化其增殖，可以在短时间内允许细胞系大量增殖。

公开(公告)号：US20100255585A1

公开(公告)日：2010-10-07

申请号：US12679263

申请日：2008-09-22

申请人： Young-Mi Yu ,  Eun Kyong Lee ,  Sun Mi Hong ,  Young Woo Jin

发明人： Young-Mi Yu ,  Eun Kyong Lee ,  Sun Mi Hong ,  Young Woo Jin

IPC分类号： C12N5/04 ,  C12N5/02

CPC分类号： C12N5/04

摘要： The present invention relates to a cell line derived from the quiescent center of a plant and a method for isolating the same, more specifically, relates to a quiescent center-derived homogeneous cell line of single-cell origin, which is obtained from the quiescent center of a plant without needing a separate de-differentiation process, and a method for isolating the same.

摘要翻译： 本发明涉及从植物的静止中心衍生的细胞系及其分离方法，更具体地涉及从静止中心获得的单细胞来源的静态中心衍生的均质细胞系 的植物，而不需要单独的去分化过程，以及用于分离其的方法。

公开(公告)号：US20080194025A1

公开(公告)日：2008-08-14

申请号：US12063929

申请日：2006-04-25

申请人： Young Woo Jin

发明人： Young Woo Jin

IPC分类号： C12N5/02

CPC分类号： C12N5/04

摘要： This invention is a method of minimizing the variation of cell growth and production through homogeneous cell line development. To be more specific, it is the method of isolating and proliferating single cell clone from the procambium or cambium to promote the stability of the plant-derived biologically active substances production by solving the problems of decrease in cell growth and the productivity during the long term culture. Key words: Procambium or cambium, single cell clone, cell aggregation, dedifferentiation, cryoperservation, variability.

摘要翻译： 本发明是通过均质细胞系发育来最小化细胞生长和产生变化的方法。 更具体地说，它是从原型或形成层分离和增殖单细胞克隆的方法，通过解决细胞生长减少和长期生产力的问题来促进植物来源的生物活性物质生产的稳定性 文化。 关键词：原核生物或形成层，单细胞克隆，细胞聚集，去分化，冷冻保护，变异性。