公开(公告)号：US07528230B2

公开(公告)日：2009-05-05

申请号：US10506925

申请日：2003-03-08

申请人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony D. Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

发明人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony D. Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

IPC分类号： C07K14/00 ,  C07H21/02 ,  C12N5/00

CPC分类号： C07K14/43595

摘要： Disclosed are mutated genes for green fluorescence proteins and enhanced inserted YFPs expressed therefrom. The mutant proteins not only maintain their fluorescence even at 37° C., but also exhibit about 20 times stronger fluorescence intensities in comparison to the conventional fluorescence proteins. Accordingly, the mutant fluorescence proteins of the present invention can be used as biosensors for detecting and analyzing the bioactivities of desired materials.

摘要翻译： 公开了绿色荧光蛋白和由其表达的增强的插入YFP的突变基因。 突变体蛋白质不仅在37℃下保持荧光，而且与常规荧光蛋白质相比也显示出强大的荧光强度的20倍。 因此，本发明的突变荧光蛋白可用作生物传感器，用于检测和分析所需材料的生物活性。

公开(公告)号：US20050170428A1

公开(公告)日：2005-08-04

申请号：US10506925

申请日：2003-03-08

申请人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

发明人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

IPC分类号： C07K14/435 ,  G01N33/53 ,  A01K67/00

CPC分类号： C07K14/43595

摘要： Disclosed are mutated genes for green fluorescence proteins and enhanced inserted YFPs expressed therefrom. The mutant proteins not only maintain their fluorescence even at 37° C., but also exhibit about 20 times stronger fluorescence intensities in comparison to the conventional fluorescence proteins. Accordingly, the mutant fluorescence proteins of the present invention can be used as biosensors for detecting and analyzing the bioactivities of desired materials.

摘要翻译： 公开了绿色荧光蛋白和由其表达的增强的插入YFP的突变基因。 突变体蛋白质不仅在37℃下保持荧光，而且与常规荧光蛋白质相比也显示出强大的荧光强度的20倍。 因此，本发明的突变荧光蛋白可用作生物传感器，用于检测和分析所需材料的生物活性。