公开(公告)号：US20050170428A1

公开(公告)日：2005-08-04

申请号：US10506925

申请日：2003-03-08

申请人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

发明人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

IPC分类号： C07K14/435 ,  G01N33/53 ,  A01K67/00

CPC分类号： C07K14/43595

摘要： Disclosed are mutated genes for green fluorescence proteins and enhanced inserted YFPs expressed therefrom. The mutant proteins not only maintain their fluorescence even at 37° C., but also exhibit about 20 times stronger fluorescence intensities in comparison to the conventional fluorescence proteins. Accordingly, the mutant fluorescence proteins of the present invention can be used as biosensors for detecting and analyzing the bioactivities of desired materials.

摘要翻译： 公开了绿色荧光蛋白和由其表达的增强的插入YFP的突变基因。 突变体蛋白质不仅在37℃下保持荧光，而且与常规荧光蛋白质相比也显示出强大的荧光强度的20倍。 因此，本发明的突变荧光蛋白可用作生物传感器，用于检测和分析所需材料的生物活性。

公开(公告)号：US07528230B2

公开(公告)日：2009-05-05

申请号：US10506925

申请日：2003-03-08

申请人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony D. Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

发明人： Dong-Seung Seen ,  Jac-Yong Park ,  Young-Shik Choi ,  Eun-Wook Choi ,  Ho-Sun Son ,  Neon-C. Jung ,  Anthony D. Kang ,  Ji-Ung Maeng ,  Kyung-Jin Kim ,  Jung-Hee Shin

IPC分类号： C07K14/00 ,  C07H21/02 ,  C12N5/00

CPC分类号： C07K14/43595

摘要： Disclosed are mutated genes for green fluorescence proteins and enhanced inserted YFPs expressed therefrom. The mutant proteins not only maintain their fluorescence even at 37° C., but also exhibit about 20 times stronger fluorescence intensities in comparison to the conventional fluorescence proteins. Accordingly, the mutant fluorescence proteins of the present invention can be used as biosensors for detecting and analyzing the bioactivities of desired materials.

摘要翻译： 公开了绿色荧光蛋白和由其表达的增强的插入YFP的突变基因。 突变体蛋白质不仅在37℃下保持荧光，而且与常规荧光蛋白质相比也显示出强大的荧光强度的20倍。 因此，本发明的突变荧光蛋白可用作生物传感器，用于检测和分析所需材料的生物活性。

公开(公告)号：US07319001B2

公开(公告)日：2008-01-15

申请号：US10383838

申请日：2003-03-07

申请人： Ho-Sun Son ,  Do-Hui Kim ,  Neon-C Jung ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Min-Sung Kim ,  Yong-Weon Yi ,  Kyung-Jin Kim

发明人： Ho-Sun Son ,  Do-Hui Kim ,  Neon-C Jung ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Min-Sung Kim ,  Yong-Weon Yi ,  Kyung-Jin Kim

IPC分类号： C12N15/10 ,  C12N15/63 ,  C12N15/64 ,  C12N15/866 ,  C12N15/86 ,  A61K48/00

CPC分类号： C12N15/86 ,  C12N15/1027 ,  C12N2710/14143

摘要： Disclosed are the methods for producing recombinant viruses using site-specific recombination in vitro. In the present invention, circular viral genomic DNAs are digested with restriction enzymes to generate a linear form viral genomic DNAs flanked by site-specific recombination sites, and then are subjected to site-specific recombination with the desired genomic materials flanked by site-specific recombination sites in vitro. According to the present invention, since the site-specific recombination mixture can be applied to host cells without further procedures of selecting the desired recombinant viral genomic DNAs, it is possible to obtain numerous recombinant viruses rapidly at the same time. Thus, the present invention can be used as a high throughput system for generating and screening hundreds or thousands of recombinant viruses.

摘要翻译： 公开了使用体外位点特异性重组生产重组病毒的方法。 在本发明中，循环病毒基因组DNA用限制性酶消化以产生侧翼为位点特异性重组位点的线性形式的病毒基因组DNA，然后进行与所需基因组物质的位点特异性重组，所述基因组物质侧接于位点特异性重组 体外试验。 根据本发明，由于可以不用进一步选择所需的重组病毒基因组DNA的方式将位点特异性重组混合物施用于宿主细胞，所以可以同时快速获得多种重组病毒。 因此，本发明可以用作产生和筛选数百或数千种重组病毒的高通量系统。