公开(公告)号：US20080039940A1

公开(公告)日：2008-02-14

申请号：US10592256

申请日：2005-02-16

申请人： Kouji Hashimoto ,  Yuuji Shirakata ,  Yuuichi Ohashi ,  Junji Hamuro

发明人： Kouji Hashimoto ,  Yuuji Shirakata ,  Yuuichi Ohashi ,  Junji Hamuro

IPC分类号： A61F2/10 ,  A01N1/00 ,  A61P43/00

CPC分类号： C12N5/0621 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3869 ,  C12M25/02 ,  C12N5/0629 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/92

摘要： A biological tissue sheet which is expected as exerting a favorable therapeutic effect and a high safety in transplantation. The biological tissue sheet formed by (a) preparing in vivo-derived cells; (b) sowing the in vivo-derived cells on amniotic membrane; and (c) culturing and proliferating the in vivo-derived cells in the absence of any xenogeneic animal cells. As the cells of a biological origin, for example, cells originating in corneal epithelium, conjunctival epithelium, skin epidermis, hair follicle epithelium, oral mucosa, respiratory tract mucosa, or intestinal tract mucosa.

摘要翻译： 预期在生物组织片中具有良好的治疗效果和高的移植安全性。 通过（a）制备体内衍生的细胞而形成的生物组织片; （b）在羊膜上播种体内衍生的细胞; 和（c）在不存在任何异种动物细胞的情况下培养和增殖体内衍生的细胞。 作为生物来源的细胞，例如来自角膜上皮，结膜上皮，皮肤表皮，毛囊上皮，口腔粘膜，呼吸道粘膜或肠道粘膜的细胞。

公开(公告)号：US20070280993A1

公开(公告)日：2007-12-06

申请号：US10592114

申请日：2005-02-14

申请人： Kouji Hashimoto ,  Yuuji Shirakata ,  Yuuichi Ohashi ,  Junji Hamuro

发明人： Kouji Hashimoto ,  Yuuji Shirakata ,  Yuuichi Ohashi ,  Junji Hamuro

IPC分类号： A61F2/14 ,  C12N5/02

CPC分类号： C12N5/0621 ,  A61L27/24 ,  A61L27/3813 ,  A61L27/3869 ,  A61L27/3895 ,  C12N2502/1323 ,  C12N2533/54

摘要： A corneal epithelial sheet having potential for the achievement of a favorable therapeutic effect and being highly safe in transplantation. The corneal epithelial sheet is constructed by: (a) preparing corneal epithelial cells; (b) separately culturing human fibroblasts in a collagen gel; (c) sowing or placing the corneal epithelial cells on the collagen gel; and (d) culturing and proliferating the corneal epithelial cells in the absence of xenogeneic animal cells.

摘要翻译： 角膜上皮片具有获得良好治疗效果和在移植中高度安全的潜力。 角膜上皮片由以下构成：（a）制备角膜上皮细胞; （b）在胶原凝胶中分别培养人成纤维细胞; （c）将角膜上皮细胞播种或放置在胶原凝胶上; 和（d）在不存在异种动物细胞的情况下培养和增殖角膜上皮细胞。