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    GENERATING CAPTURE PROBES FOR SPATIAL ANALYSIS
    1.
    发明申请
    GENERATING CAPTURE PROBES FOR SPATIAL ANALYSIS 有权

    公开(公告)号：US20230002812A1

    公开(公告)日：2023-01-05

    申请号：US17776769

    申请日：2020-11-13

    申请人： 10x Genomics, Inc.

    发明人： Marlon Stoeckius ,  Stefania Giacomello ,  Zachary Bent ,  Meghan L. F. Frey ,  Alexander Gagnon ,  Erik Leonard Henrik Borgstrom

    IPC分类号： C12Q1/6837

    摘要： The present disclosure relates to compositions, methods, and kits for generating capture probes on a substrate for identifying the location of analytes in a biological sample. In particular, disclosed is a method of generating a spatial array comprising: (a) providing a substrate comprising a plurality of acceptor oligonucleotides, wherein an acceptor oligonucleotide of the plurality of acceptor oligonucleotides comprises a spatial barcode and a first ligation handle, and wherein the 5′ end of the acceptor oligonucleotide is attached to the substrate; (b) providing a plurality of universal splint oligonucleotides, wherein a universal splint oligonucleotide of the plurality of universal splint oligonucleotides comprises a sequence complementary to the first ligation handle and a sequence complementary to a second ligation handle present in a donor oligonucleotide of a plurality of donor oligonucleotides; and (c) ligating the donor oligonucleotide comprising a capture domain to the 3′ end of the acceptor oligonucleotide to generate a capture probe, wherein the universal splint oligonucleotide is hybridized to the first ligation handle and the second ligation handle, thereby generating a spatial array.