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公开(公告)号:US20220403455A1
公开(公告)日:2022-12-22
申请号:US17891641
申请日:2022-08-19
申请人: 10x Genomics, Inc.
IPC分类号: C12Q1/6837 , C12Q1/6841
摘要: Provided herein are methods of determining a location of a target analyte in a non-permeabilized biological sample that include the use of a blocking probe.
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公开(公告)号:US20210324457A1
公开(公告)日:2021-10-21
申请号:US17271866
申请日:2019-08-27
申请人: Eswar Prasad RAMACHANDRAN IYER , Michael SCHNALL-LEVIN , Augusto Manuel TENTORI , Tarje Sigurd MIKKELSEN , Benjamin HINDSON , Michael Ybarra LUCERO , Rajiv BHARADWAJ , Yifeng YIN , Zachary BENT , Jennifer CHEW , Meghan L. F. FREY , Linda KVASTAD , Joakim LUNDEBERG , Eva Gracia VILLACAMPA , 10x Genomics, Inc.
发明人: Eswar Prasad Ramachandran Iyer , Michael Schnall-Levin , Augusto Manuel Tentori , Tarjei Sigurd Mikkelsen , Benjamin Hindson , Michael Ybarra Lucero , Rajiv Bharadwaj , Yifeng Yin , Zachary Bent , Jennifer Chew , Meghan L. F. Frey , Linda Kvastad , Joakim Lundeberg , Eva Gracia Villacampa
IPC分类号: C12Q1/6837 , C12Q1/6841
摘要: This disclosure relates to methods for generating spatially barcoded arrays using concentration gradients.
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公开(公告)号:US20210155982A1
公开(公告)日:2021-05-27
申请号:US16951864
申请日:2020-11-18
申请人: 10X Genomics, Inc.
发明人: Yifeng Yin , Zachary Bent , Stephen Williams , Ian Fiddes , Jeffrey Clark Mellen , Jasper Staab , Kevin J. Wu , Neil Ira Weisenfeld , Florian Baumgartner , Brynn Claypoole , Preyas Shah , Narek Dshkhunyan , Erik Leonard Henrik Borgstrom , Benjamin McCreath
IPC分类号: C12Q1/6869 , C12Q1/6837 , G06T7/194 , G06T7/13 , G06T7/00 , G06N5/00
摘要: Systems and methods for spatial analysis of analytes include placing a sample on a substrate having fiducial markers and capture spots. Then, an image of the sample is acquired and sequence reads are obtained from the capture spots. Each capture probe plurality in a set of capture probe pluralities is (i) at a different capture spot, (ii) directly or indirectly associates with analytes from the sample and (iii) has a unique spatial barcode. The sequencing reads serve to detect the analytes. Sequencing reads include a spatial barcode of the corresponding capture probe plurality. Spatial barcodes localize reads to corresponding capture spots, thereby dividing them into subsets, each subset for a respective capture spot. Fiducial markers facilitate a composite representation comprising (i) the image aligned to the capture spots and (ii) a representation of each subset of sequence reads at respective positions within the image mapping to the corresponding capture spots.
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公开(公告)号:US20210102936A1
公开(公告)日:2021-04-08
申请号:US16897126
申请日:2020-06-09
申请人: 10X Genomics, Inc.
发明人: Zahra Kamila Belhocine , Jason Bell , Zachary Bent , Rajiv Bharadwaj , Christopher Hindson , Mohammad Rahimi Lenji , Bill Lin , Anthony Makarewicz , Geoffrey McDermott , Elliott Meer , Francesca Meschi , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Michael Schnall-Levin , Sarah Taylor , Jessica Michele Terry , Tobias Daniel Wheeler , Yifeng Yin , Xinying Zheng , Solongo Batjargal Ziraldo , Eswar Prasad Ramachandran Iyer , Luigi Jhon Alvarado Martinez
IPC分类号: G01N33/532 , C07K14/74 , C12N15/85 , G01N33/543 , G01N33/548 , C12N15/10 , C12Q1/6804 , C12Q1/6806 , G01N33/50 , G01N33/53 , G01N33/58 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , C12N15/11 , G01N33/569
摘要: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).
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公开(公告)号:US20200140944A1
公开(公告)日:2020-05-07
申请号:US16692631
申请日:2019-11-22
申请人: 10X GENOMICS, INC.
发明人: Phillip Belgrader , Zachary Bent , Rajiv Bharadwaj , Vijay Kumar Sreenivasa Gopalan , Josephine Harada , Christopher Hindson , Mohamad Rahimi Lenji , Michael Ybarra Lucero , Geoffrey McDermott , Elliott Meer , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Serge Saxonov , John R. Stuelpnagel , Jessica Michele Terry , Tobias Daniel Wheeler , Indira Wu , Solongo Batjargal Ziraldo
IPC分类号: C12Q1/6874 , C12N15/10 , C12Q1/683 , C12Q1/6804 , C12Q1/6806
摘要: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
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公开(公告)号:US20200063191A1
公开(公告)日:2020-02-27
申请号:US16680343
申请日:2019-11-11
申请人: 10X GENOMICS, INC.
发明人: Zachary Bent , Elliott Meer , Daniel Riordan , Paul Ryvkin , Niranjan Srinivas , Jessica Terry , Alex Gagnon
IPC分类号: C12Q1/6806 , G06K19/06 , C40B50/18 , C40B50/16 , C40B20/04 , C12Q1/6834 , C12Q1/6811
摘要: The present disclosure provides methods of generating supports (e.g., beads) comprising barcode molecules coupled thereto. A barcode molecule coupled to a support may comprise a barcode sequence and a functional sequence. A barcode molecule may be generated using two or more ligation reactions in a combinatorial fashion. A support comprising two or more different barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.
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公开(公告)号:US20190383798A1
公开(公告)日:2019-12-19
申请号:US16375110
申请日:2019-04-04
申请人: 10X Genomics, Inc.
发明人: Kamila Belhocine , Jason Bell , Zachary Bent , Rajiv Bharadwaj , Christopher Hindson , Mohammad Rahimi Lenji , Bill Kengli Lin , Anthony Makarewicz , Geoffrey McDermott , Elliott Meer , Francesca Meschi , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Michael Schnall-Levin , Sarah Taylor , Jessica Michele Terry , Tobias Daniel Wheeler , Yifeng Yin , Xinying Zheng , Solongo Batjargal Ziraldo , Eswar Prasad Ramachandran Iyer , Luigi Jhon Alvarado Martinez
IPC分类号: G01N33/50 , G01N33/543 , G01N33/53 , G01N33/569 , C12N15/10
摘要: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.
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公开(公告)号:US20220098661A1
公开(公告)日:2022-03-31
申请号:US17548047
申请日:2021-12-10
申请人: 10x Genomics, Inc.
IPC分类号: C12Q1/6874 , C12Q1/6825
摘要: Provided herein are methods for resetting an array to which a biological sample has been applied that include treating the array with a set of biological sample removal conditions.
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公开(公告)号:US20210372998A1
公开(公告)日:2021-12-02
申请号:US17209045
申请日:2021-03-22
申请人: 10X Genomics, Inc.
发明人: Zahra Kamila Belhocine , Jason Bell , Zachary Bent , Rajiv Bharadwaj , Christopher Hindson , Mohammad Rahimi Lenji , Bill Kengli Lin , Anthony Makarewicz , Geoffrey McDermott , Elliott Meer , Francesca Meschi , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Michael Schnall-Levin , Sarah Taylor , Jessica Michele Terry , Tobias Daniel Wheeler , Yifeng Yin , Xinying Zheng , Solongo Batjargal Ziraldo , Eswar Prasad Ramachandran Iyer , Luigi Jhon Alvarado Martinez
IPC分类号: G01N33/532 , C07K14/74 , C12N15/85 , G01N33/543 , G01N33/548 , C12N15/10 , C12Q1/6804 , C12Q1/6806 , G01N33/50 , G01N33/53 , G01N33/58 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , C12N15/11 , G01N33/569
摘要: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.
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公开(公告)号:US20210285036A1
公开(公告)日:2021-09-16
申请号:US17212681
申请日:2021-03-25
申请人: 10x Genomics, Inc.
发明人: Yifeng Yin , Eswar Prasad Ramachandran Iyer , Zachary Bent , Stefania Giacomello , Meghan L. F. Frey , Alexander Gagnon , Erik Leonard Henrik Borgstrom
IPC分类号: C12Q1/6837
摘要: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.
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