公开(公告)号：US20240331348A1

公开(公告)日：2024-10-03

申请号：US18737862

申请日：2024-06-07

Applicant: 10x Genomics, Inc.

Inventor： Felice Alessio BAVA ,  David Hoffman ,  Elijah Roberts

IPC: G06V10/60 ,  G06T3/40 ,  G06T7/70 ,  G06V10/10 ,  G06V20/69

CPC classification number: G06V10/60 ,  G06T3/40 ,  G06T7/70 ,  G06V10/19 ,  G06V20/693

Abstract: Methods and systems for performing multi-resolution in situ decoding are described. The method may comprise, for example, acquiring at least one first image of a biological sample at a first optical resolution; identifying locations for a plurality of target analytes based on the at least one first image; acquiring at least one second image of the biological sample at a second optical resolution in at least one decoding cycle of a plurality of decoding cycles used for in situ decoding of the target analytes; and extracting signal intensity data for signals associated with all or a portion of the target analytes from the at least one second image based on the locations for the target analytes identified in the at least one first image. In some instances, the method may further comprise using the signal intensity data extracted from the at least one second image to decode the target analytes.

公开(公告)号：US20240305314A1

公开(公告)日：2024-09-12

申请号：US18660149

申请日：2024-05-09

Applicant: 10x Genomics, Inc.

Inventor： David HOFFMAN ,  Denis Pristinski ,  Elijah Roberts ,  Preyas Shah

IPC: H03M13/37 ,  G01N21/64

CPC classification number: H03M13/3753 ,  G01N21/6456 ,  G01N2021/6471

Abstract: Techniques for spectral unmixing and decoding for in situ analysis are provided. Input hypercube data comprising voxel data, raw channel data, and decoding round data are obtained, and an initial iteration of codeword data determination is performed. Performing the initial iteration includes generating initial feature data and initial unmixed fluorescence data, generating initial uncorrected codeword data, and generating initial corrected codeword data. One or more subsequent iterations of codeword data determination are then performed based on the input hypercube data and based on feature data, unmixed fluorescence data, uncorrected codeword data, and corrected codeword data from one or more previous iterations. When it is determined that one or more convergence conditions have been satisfied, output comprising an optimized estimate of fluorescence data, and an optimized estimate of uncorrected codeword data, and an optimized estimate of corrected codeword data is generated.

公开(公告)号：US20250117932A1

公开(公告)日：2025-04-10

申请号：US18907230

申请日：2024-10-04

Applicant: 10x Genomics, Inc.

Inventor： Jeffrey Mellen ,  Jeffrey Peck ,  Edward Jaesung Kim DePuy ,  Andreas Girgensohn ,  Paul Ryvkin ,  Elijah Roberts

IPC: G06T7/00

Abstract: Feature pyramiding for in situ data visualizations is provided. In various embodiments, a point cloud is loaded. Each point within the point cloud represents a feature of a biological sample at its corresponding location. At least one tile is determined having a two-dimensional area and a zoom level. Those points having a location within the two-dimensional area are selected from the point cloud. Based on the selected points and the zoom level, a set of display points is determined, each point of the set of display point representing a feature of the biological sample at its corresponding location. The set of display points is provided for display at the zoom level.