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公开(公告)号：US20230160794A1
公开(公告)日：2023-05-25
申请号：US17993330
申请日：2022-11-23
Applicant: 10x Genomics, Inc.
Inventor： Rhyan Blaine DOCKTER , David HOFFMAN
CPC classification number: G01N1/312 , G01N21/6458 , G02B21/34 , G06T7/0012 , B01L2300/021 , B01L2300/0822
Abstract: The present disclosure relates to methods for preparing biological samples for in situ analysis of one or more analytes wherein the biological sample has been previously affixed to a substrate, which in some cases may not be compatible with in situ analysis, for example, due to the absence of positional markers and/or fiducial markers and/or a region suitable for in situ signal detection on the substrate. In some aspects, a hydrophobic adapter having positional markers and/or fiducial markers is applied to the substrate to which a biological sample has been affixed, thus enabling in situ sample processing and analysis of the biological sample. The hydrophobic adhesive label or adapter can be used for automated microscope alignment and sample preparation.
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公开(公告)号：US20240167956A1
公开(公告)日：2024-05-23
申请号：US18509689
申请日：2023-11-15
Applicant: 10x Genomics, Inc.
Inventor： David HOFFMAN , Denis Pristinski , Evan DeJrnette , David Morgan
IPC: G01N21/64
CPC classification number: G01N21/6456 , G01N21/6428 , G01N2021/6439 , G01N2201/062 , G01N2201/0636
Abstract: An imaging system includes an optical mounting plate having an aperture disposed between a first portion and a second portion, an imaging sensor disposed on the first portion, an objective lens disposed on the second portion, and an illumination assembly arranged within the aperture and disposed between the imaging sensor and the objective lens. The imaging system includes a heat sink coupled to the illumination assembly and configured to transfer heat away from light sources of the illumination assembly via a working fluid that flows through the heat sink.
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Patent Agency Ranking
公开(公告)号：US20240305314A1
公开(公告)日：2024-09-12
申请号：US18660149
申请日：2024-05-09
Applicant: 10x Genomics, Inc.
Inventor： David HOFFMAN , Denis Pristinski , Elijah Roberts , Preyas Shah
CPC classification number: H03M13/3753 , G01N21/6456 , G01N2021/6471
Abstract: Techniques for spectral unmixing and decoding for in situ analysis are provided. Input hypercube data comprising voxel data, raw channel data, and decoding round data are obtained, and an initial iteration of codeword data determination is performed. Performing the initial iteration includes generating initial feature data and initial unmixed fluorescence data, generating initial uncorrected codeword data, and generating initial corrected codeword data. One or more subsequent iterations of codeword data determination are then performed based on the input hypercube data and based on feature data, unmixed fluorescence data, uncorrected codeword data, and corrected codeword data from one or more previous iterations. When it is determined that one or more convergence conditions have been satisfied, output comprising an optimized estimate of fluorescence data, and an optimized estimate of uncorrected codeword data, and an optimized estimate of corrected codeword data is generated.
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公开(公告)号：US20240159292A1
公开(公告)日：2024-05-16
申请号：US18505635
申请日：2023-11-09
Applicant: 10x Genomics, Inc.
Inventor： Vu Huy NGUYEN , Denis PRISTINSKI , David MORGAN , Evan DEJARNETTE , Tyler DRAKE , David HOFFMAN
IPC: F16F15/027
CPC classification number: F16F15/027 , F16F2228/04
Abstract: Various embodiments of the present disclosure disclose mitigating internal and external vibrational disturbances in opto-fluidic instrument. In various embodiments, the resonance frequency ranges and/or the vibrational frequency ranges of various modules and components of the opto-fluidic instrument may be selected to avoid resonances from occurring in the opto-fluidic instrument, thereby mitigating vibrations from internal and external sources that may distort the imaging of samples in the opto-fluidic instrument.
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公开(公告)号：US20240369471A1
公开(公告)日：2024-11-07
申请号：US18775876
申请日：2024-07-17
Applicant: 10x Genomics, Inc.
Inventor： David HOFFMAN , Denis PRISTINSKI , Rajiv BHARADWAJ , Yiran ZHANG
Abstract: Disclosed are assemblies, systems, and methods for transilluminating a sample disposed within a device. An assembly includes a first optically transparent substrate configured to receive a biological sample, a second optically transparent substrate having a top surface, a bottom surface, and a plurality of sides. The first substrate contacts the top surface of the second substrate. The assembly includes at least one light source configured to illuminate at least one of the plurality of sides of the second substrate and a light scattering layer on the bottom surface of the second substrate. The light scattering layer is configured to scatter light from the light source. The assembly includes a thermal control module coupled to the second substrate and configured to control the temperature of the second substrate.
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公开(公告)号：US20240171833A1
公开(公告)日：2024-05-23
申请号：US18509584
申请日：2023-11-15
Applicant: 10x Genomics, Inc.
Inventor： David HOFFMAN , Denis PRISTINSKI , Evan DeJARNETTE , David MORGAN
Abstract: An imaging system includes an optical mounting plate having an aperture disposed between a first portion and a second portion, an imaging sensor disposed on the first portion, an objective lens disposed on the second portion, and an illumination assembly arranged within the aperture and disposed between the imaging sensor and the objective lens. The imaging system includes a heat sink coupled to the illumination assembly and configured to transfer heat away from light sources of the illumination assembly via a working fluid that flows through the heat sink.
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7.发明公开公开(公告)号：US20230314327A1
公开(公告)日：2023-10-05
申请号：US18130820
申请日：2023-04-04
Applicant: 10x Genomics, Inc.
Inventor： David HOFFMAN
IPC: G01N21/64
CPC classification number: G01N21/6456 , G01N21/6428 , G01N2021/6439
Abstract: A tilted structured illumination pattern for use in an optical system is generated, wherein the tilted structured illumination pattern is not symmetrical about a center point of a back pupil of the system, and wherein the tilted structured illumination pattern is based on exactly two wave vectors and defines spatial frequencies along an optical axis of an objective of the system. The tilted structured illumination pattern is directed to be incident on a sample, such that one or more fluorophores of the sample are excited by the structured illumination pattern. A phase of the tilted structured illumination pattern is varied. A set of images of fluorescence emission emitted by the sample is captured, wherein each image of the set of images corresponds to a respective phase of the tilted structured illumination pattern and to a respective axial imaging plane. Combined image data is generated based on the set of images.
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