公开(公告)号：US20230278038A1

公开(公告)日：2023-09-07

申请号：US18177562

申请日：2023-03-02

Applicant: 10x Genomics, Inc.

Inventor： Mohammad RAHIMI LENJI ,  Yiran ZHANG ,  Francis MARCOGLIESE ,  Tobias Daniel WHEELER ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011

CPC classification number: B01L3/502792 ,  B01F23/4144 ,  B01F33/3011 ,  B01F23/4105 ,  B01L2300/0867 ,  B01L2200/16 ,  B01L2200/0673 ,  B01L2300/0816

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. Devices use flow focusing arrangements of channels to produce droplets. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20230278031A1

公开(公告)日：2023-09-07

申请号：US18154516

申请日：2023-01-13

Applicant: 10x Genomics, Inc.

Inventor： Francis MARCOGLIESE ,  Yiran ZHANG

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/523 ,  B01L3/527 ,  B01L3/502738 ,  B01L2200/16 ,  B01L2300/021

Abstract: Devices, systems, and methods for directing fluid flow to one or more specific regions of interest within a closed flow cell are provided. A device includes a first directing channel having a first directing proximal portion and a first directing distal portion, a second directing channel having a second directing proximal portion and a second directing distal portion, an inlet channel having an inlet proximal portion and an inlet distal portion, a reaction chamber, and a waste outlet. The inlet distal portion is disposed between the first directing distal portion and the second directing distal portion. The first directing distal portion, the second directing distal portion, and the inlet channel may be substantially parallel. The first directing channel, the second directing channel, the first reagent channel, and the waste outlet are in fluid communication with the reaction chamber.