公开(公告)号：US20240165626A1

公开(公告)日：2024-05-23

申请号：US18507195

申请日：2023-11-13

Applicant: 10x Genomics, Inc.

Inventor： Eric EVJE ,  Xueda SHI ,  Yiran ZHANG

IPC: B01L3/00 ,  G01N35/10

CPC classification number: B01L3/52 ,  G01N35/1002 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/16 ,  B01L2300/0809 ,  B01L2300/161 ,  G01N2021/6439

Abstract: Various embodiments of the present disclosure disclose methods and systems for dispensing a reagent into a sample well and efficiently extracting the dispensed reagent from the sample well after the reagent is used for detecting molecules in a sample. The amount of probe reagents dispensed may be no more than what is needed to immerse the sample and allow effective probing of the sample by the probes in the reagent.

公开(公告)号：US20230324421A1

公开(公告)日：2023-10-12

申请号：US18132115

申请日：2023-04-07

Applicant: 10x Genomics, Inc.

Inventor： Yiran ZHANG ,  Felice Alessio BAVA ,  Rhyan Blaine DOCKTER ,  Rajiv BHARADWAJ

IPC: G01N35/00 ,  B01L3/00

CPC classification number: G01N35/00029 ,  B01L3/502715 ,  G01N2035/00168 ,  B01L2200/0689 ,  B01L2200/025 ,  B01L2300/0627 ,  B01L2200/16

Abstract: Disclosed herein are systems for receiving a biological sample for analysis and methods for preparing and/or analyzing a biological sample. In various embodiments, a system includes a first layer comprising a first region configured to receive a sample, a gasket disposed on the first layer such that the gasket surrounds the first region, and a housing layer. The first layer is disposed between the gasket and the housing layer. The system further includes a clamp configured to apply a force on the gasket to form a liquid tight seal against the first layer.

公开(公告)号：US20230167489A1

公开(公告)日：2023-06-01

申请号：US18070887

申请日：2022-11-29

Applicant: 10x Genomics, Inc.

Inventor： Yiran ZHANG ,  Siyuan XING ,  Rajiv BHARADWAJ

IPC: C12Q1/6841 ,  C12Q1/6837

CPC classification number: C12Q1/6841 ,  C12Q1/6837

Abstract: The present invention features devices and methods for supplying a biological sample with a fluid. In various embodiments, a device includes a first layer and a second layer. The first layer includes a window having a thickness of about 1 μm to about 1000 μm. The first and second layers reversibly seal to form a flow path having an inlet and an outlet and bounded in part by the window.

公开(公告)号：US20220080424A1

公开(公告)日：2022-03-17

申请号：US17357617

申请日：2021-06-24

Applicant: 10X Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Francis CUI ,  Rachel GERVER ,  Mohammad Rahimi LENJI ,  Bill Kengli LIN ,  Anthony MAKAREWICZ ,  Martin SAUZADE ,  Astha TANNA ,  Fernandino VALDECANAS ,  Tobias Daniel WHEELER ,  Yiran ZHANG ,  Alireza SALMANZADEH

IPC: B01L3/00 ,  B01L7/00

Abstract: Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.

公开(公告)号：US20240369471A1

公开(公告)日：2024-11-07

申请号：US18775876

申请日：2024-07-17

Applicant: 10x Genomics, Inc.

Inventor： David HOFFMAN ,  Denis PRISTINSKI ,  Rajiv BHARADWAJ ,  Yiran ZHANG

IPC: G01N21/03 ,  G01N21/05 ,  G01N21/47 ,  G01N21/64

Abstract: Disclosed are assemblies, systems, and methods for transilluminating a sample disposed within a device. An assembly includes a first optically transparent substrate configured to receive a biological sample, a second optically transparent substrate having a top surface, a bottom surface, and a plurality of sides. The first substrate contacts the top surface of the second substrate. The assembly includes at least one light source configured to illuminate at least one of the plurality of sides of the second substrate and a light scattering layer on the bottom surface of the second substrate. The light scattering layer is configured to scatter light from the light source. The assembly includes a thermal control module coupled to the second substrate and configured to control the temperature of the second substrate.

公开(公告)号：US20230347312A1

公开(公告)日：2023-11-02

申请号：US18131159

申请日：2023-04-05

Applicant: 10x Genomics, Inc.

Inventor： Yiran ZHANG ,  Siyuan XING ,  Denis PRISTINSKI

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2204/00 ,  B01J2219/00416 ,  B01J2219/00596 ,  B01J2219/00632 ,  B01J2219/00722 ,  B01J2219/00831 ,  B01J2219/1233

Abstract: Systems and methods for dispensing a liquid on a substrate are disclosed. The system may be used to coat a substrate and/or a sample (e.g., a biological sample) with a liquid. The system includes a rotating device configured to rotate about an axis and a substrate disposed on the rotating device. The substrate comprises a well configured to hold a liquid and the substrate is positioned on a surface of the rotating device perpendicular to and concentric with the axis. The system further includes a liquid handler configured to dispense the liquid into the well. The rotating device is configured to rotate at a speed sufficient to disperse the liquid within the well.

公开(公告)号：US20210387195A1

公开(公告)日：2021-12-16

申请号：US17458757

申请日：2021-08-27

Applicant: 10x Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Mohammad Rahimi LENJI ,  Alireza SALMANZADEH ,  Martin SAUZADE ,  Tobias Daniel WHEELER ,  Yiran ZHANG

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08 ,  B01F5/04

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20230312209A1

公开(公告)日：2023-10-05

申请号：US18131223

申请日：2023-04-05

Applicant: 10x Genomics, Inc.

Inventor： David MORGAN ,  Evan DEJARNETTE ,  Eric EVJE ,  Yiran ZHANG ,  Siyuan XING

IPC: B65D75/36

CPC classification number: B65D75/36 ,  B65D2575/3218

Abstract: Systems that include blister packs and methods of use thereof are disclosed. In various embodiments, a system includes a blister pack having at least one blister comprising a base and a top layer. The blister contains a liquid reagent. The system further includes an actuator configured to release the liquid reagent from the blister. The system further includes a substrate configured to hold a tissue sample and receive the liquid reagent from the blister upon release.

公开(公告)号：US20230278038A1

公开(公告)日：2023-09-07

申请号：US18177562

申请日：2023-03-02

Applicant: 10x Genomics, Inc.

Inventor： Mohammad RAHIMI LENJI ,  Yiran ZHANG ,  Francis MARCOGLIESE ,  Tobias Daniel WHEELER ,  Rajiv BHARADWAJ

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011

CPC classification number: B01L3/502792 ,  B01F23/4144 ,  B01F33/3011 ,  B01F23/4105 ,  B01L2300/0867 ,  B01L2200/16 ,  B01L2200/0673 ,  B01L2300/0816

Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. Devices use flow focusing arrangements of channels to produce droplets. The invention further provides multiplex devices that increase droplet formation.

公开(公告)号：US20230278031A1

公开(公告)日：2023-09-07

申请号：US18154516

申请日：2023-01-13

Applicant: 10x Genomics, Inc.

Inventor： Francis MARCOGLIESE ,  Yiran ZHANG

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/523 ,  B01L3/527 ,  B01L3/502738 ,  B01L2200/16 ,  B01L2300/021

Abstract: Devices, systems, and methods for directing fluid flow to one or more specific regions of interest within a closed flow cell are provided. A device includes a first directing channel having a first directing proximal portion and a first directing distal portion, a second directing channel having a second directing proximal portion and a second directing distal portion, an inlet channel having an inlet proximal portion and an inlet distal portion, a reaction chamber, and a waste outlet. The inlet distal portion is disposed between the first directing distal portion and the second directing distal portion. The first directing distal portion, the second directing distal portion, and the inlet channel may be substantially parallel. The first directing channel, the second directing channel, the first reagent channel, and the waste outlet are in fluid communication with the reaction chamber.