公开(公告)号：US20240360494A1

公开(公告)日：2024-10-31

申请号：US18763579

申请日：2024-07-03

Applicant: 10x Genomics, Inc.

Inventor： Justin Costa ,  Felice Alessio Bava

IPC: C12Q1/6837 ,  C12Q1/6876

CPC classification number: C12Q1/6837 ,  C12Q1/6876

Abstract: Provided herein are methods, compositions, and kits for capturing a ligation product, in which the method can include covalently reacting a capture moiety of a capture probe with a reactive moiety of the ligation product to form a captured ligation product.

公开(公告)号：US20250163506A1

公开(公告)日：2025-05-22

申请号：US18955558

申请日：2024-11-21

Applicant: 10x Genomics, Inc.

Inventor： Marco Serra ,  Justin Costa

IPC: C12Q1/6874 ,  C12Q1/44 ,  C12Q1/6806

Abstract: Systems and methods for the enhanced spatial analysis of interactions between nucleic acids and proteins in a biological sample have been developed. The methods employ a first set of nucleic acid-based probes to selectively bind target proteins in a biological sample and functionalize the nucleic acids within the sample to enable embedding within a permeable matrix, such as a gel. The methods then implement probe-based spatial analysis within the permeable matrix using a second set of probes designed to hybridize with both a target nucleic acid and with the first probes bound to the target proteins. The resulting hybridized first and second probes are bound by a capture probe including a spatial recognition barcode to provide spatial information for interactions between nucleic acids and target proteins within the sample.

公开(公告)号：US20250066770A1

公开(公告)日：2025-02-27

申请号：US18948720

申请日：2024-11-15

Applicant: 10x Genomics, Inc.

Inventor： Justin Costa

IPC: C12N15/10

Abstract: Disclosed herein are modified split mirrored transposons or double-stranded split mirrored transposon (SMT) compositions and methods of using SMTs. The methods include producing SMTs and using SMTs for nucleic acid analysis.