公开(公告)号：US20220218814A1

公开(公告)日：2022-07-14

申请号：US17609822

申请日：2020-05-08

Applicant: ACADEMIA SINICA ,  NATIONAL TAIWAN UNIVERSITY

Inventor： Che-Ming Jack HU ,  Hung-Chih YANG

IPC: A61K39/12 ,  A61K39/39 ,  A61K9/51

Abstract: We utilized a biocompatible hollow polymeric nanoparticle that coencapsulates T cell epitope peptides and oligodeoxynucleotide (ODN) CpG, and designed immunization strategies to evaluate its protectivity against influenza viruses in mice. This nanoparticle-based peptide vaccine adjuvanted with CpG stimulated robust antigen-specific CD4 and CD5 T cell immunity, but only caused minimal adverse effects compared with crude mixture of peptides and CpG. We used two peptides derived from the nucleocapsid protein (NP), MHC class I-restricted NP366-374 and MHC class ll-restricted NP311-325. This novel nanoparticle vaccine with two epitope peptides plus CpG induced robust and fully protective T cell immunity against influenza viruses. We demonstrates the utility of this novel hollow nanoparticle with co-encapsulation of only a pair of CD4+ and CD8+ T cell-stimulating influenza viral peptides and CpG in establishing near-sterilizing protective resident T cell immunity against heterosubtypic IAV infections, a critical step towards the development of universal influenza T cell vaccines.

公开(公告)号：US20230094267A1

公开(公告)日：2023-03-30

申请号：US17908585

申请日：2021-03-02

Applicant: ACADEMIA SINICA

Inventor： Che-Ming Jack HU ,  Shih-Yu CHEN ,  Yi-Fu WANG ,  Wan-Chen HSIEH ,  Yi-Shiuan TZENG ,  Ya-Ting LU ,  Jung-Chen LIN ,  Chung-Yao HSU

IPC: C12N5/0783 ,  C07K14/705 ,  C07K14/54

Abstract: A method of expanding natural killer cells, comprising: providing a population of internally gelated cells, each of which includes a gelated interior and a fluid cell membrane that contains one or more membrane-bound proteins each or collectively are capable of stimulating expansion of natural killer (NK) cells; and culturing a population of cells containing NK cells, which are capable of responding to the one or more membrane-bound proteins, with the population of internally gelated cells under conditions that allow expansion of NK cells.

公开(公告)号：US20210361765A1

公开(公告)日：2021-11-25

申请号：US16959758

申请日：2019-01-03

Applicant: ACADEMIA SINICA

Inventor： Che-Ming Jack HU ,  Saborni CHATTOPADHYAY

IPC: A61K39/39 ,  A61K9/51

Abstract: This disclosure provides treatment kit that are capable of modulating the immune response. The treatment kit may also be used enhance the immunogenicity of antigens released from cell debris. Also provided are methods of using the treatment kit.

公开(公告)号：US20190170745A1

公开(公告)日：2019-06-06

申请号：US16322014

申请日：2017-07-28

Applicant: Che-Ming Jack HU ,  ACADEMIA SINICA

Inventor： Che-Ming Jack HU ,  Hui-Wen CHEN ,  Yuan-I CHEN ,  Chen-Ying CHEIN ,  Jung-Chen LIN

IPC: G01N33/556 ,  G01N33/543 ,  C12N5/078

Abstract: A method of generating an internally fixed lipid vesicle, comprising: providing a precursor lipid vesicle that contains an aqueous interior enclosed by a lipid membrane, wherein the lipid membrane of the precursor lipid vesicle is non-permeable to a crosslinker; permeabilizing the lipid membrane transiently to generate a permeable vesicle; contacting the permeable vesicle with an inactive activatable crosslinker, whereby the inactive activatable crosslinker enters the permeable vesicle; allowing the permeable vesicle to return to a non-permeable vesicle; removing any extravesicular crosslinker; and activating the inactive activatable crosslinker to allow crosslinking to occur inside the non-permeable vesicle, whereby an internally fixed lipid vesicle is generated.