公开(公告)号：US20230094267A1

公开(公告)日：2023-03-30

申请号：US17908585

申请日：2021-03-02

Applicant: ACADEMIA SINICA

Inventor： Che-Ming Jack HU ,  Shih-Yu CHEN ,  Yi-Fu WANG ,  Wan-Chen HSIEH ,  Yi-Shiuan TZENG ,  Ya-Ting LU ,  Jung-Chen LIN ,  Chung-Yao HSU

IPC: C12N5/0783 ,  C07K14/705 ,  C07K14/54

Abstract: A method of expanding natural killer cells, comprising: providing a population of internally gelated cells, each of which includes a gelated interior and a fluid cell membrane that contains one or more membrane-bound proteins each or collectively are capable of stimulating expansion of natural killer (NK) cells; and culturing a population of cells containing NK cells, which are capable of responding to the one or more membrane-bound proteins, with the population of internally gelated cells under conditions that allow expansion of NK cells.

公开(公告)号：US20190170745A1

公开(公告)日：2019-06-06

申请号：US16322014

申请日：2017-07-28

Applicant: Che-Ming Jack HU ,  ACADEMIA SINICA

Inventor： Che-Ming Jack HU ,  Hui-Wen CHEN ,  Yuan-I CHEN ,  Chen-Ying CHEIN ,  Jung-Chen LIN

IPC: G01N33/556 ,  G01N33/543 ,  C12N5/078

Abstract: A method of generating an internally fixed lipid vesicle, comprising: providing a precursor lipid vesicle that contains an aqueous interior enclosed by a lipid membrane, wherein the lipid membrane of the precursor lipid vesicle is non-permeable to a crosslinker; permeabilizing the lipid membrane transiently to generate a permeable vesicle; contacting the permeable vesicle with an inactive activatable crosslinker, whereby the inactive activatable crosslinker enters the permeable vesicle; allowing the permeable vesicle to return to a non-permeable vesicle; removing any extravesicular crosslinker; and activating the inactive activatable crosslinker to allow crosslinking to occur inside the non-permeable vesicle, whereby an internally fixed lipid vesicle is generated.