公开(公告)号：US20190277843A1

公开(公告)日：2019-09-12

申请号：US16365579

申请日：2019-03-26

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Chung-YI WU ,  Susan Y. TSENG

IPC: G01N33/553 ,  G01N33/58 ,  C40B50/14 ,  C40B40/12

Abstract: Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and the slides are more sensitive than transparent glass slides in binding analysis.

公开(公告)号：US20170038378A1

公开(公告)日：2017-02-09

申请号：US14182290

申请日：2014-02-18

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Susan Y. TSENG

IPC: G01N33/553 ,  G01N33/58

CPC classification number: G01N33/553 ,  C40B40/12 ,  C40B50/14 ,  G01N33/582 ,  G01N2333/942 ,  G01N2400/10 ,  H01J49/40

Abstract: Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis.

Abstract translation: 镀铝玻璃片提供了一种新的聚糖阵列平台。 具体来说，铝涂层玻璃片增加了基于荧光的测定方法的灵敏度。 另外，铝涂层玻璃载玻片允许碳水化合物的质谱分析，并提供一个检查纤维素酶活性的平台。 ACG载玻片的独特性质包括：1）表面上的金属氧化物层可以活化，用于接枝有机化合物如改性寡糖; 2）表面保持导电，并且接枝的寡糖可以通过质谱法和碳水化合物结合测定同时表征; 和3）在结合分析中，载玻片比透明玻片更敏感。