-
公开(公告)号：US20220235350A1
公开(公告)日：2022-07-28
申请号：US17627747
申请日：2019-11-19
发明人： Shengqi WANG , Rui XIAO , Hongjuan WEI , Zhen RONG , Liyan LIU
摘要： The present application belongs to the technical field of mRNA preparation, and discloses a system and a method for preparing mRNA, which can shorten a preparation period, and avoid degradation of an mRNA product due to the introduction of RNase by manual pipetting. The system for preparing mRNA comprises a PCR amplification device configured to amplify DNA; a plurality of raw reagent tubes, each of which is configured to provide a single reagent; and a reaction device configured to allow reagents to react. Specifically, the reaction device comprises a test tube rack configured to support reaction tubes, a semiconductor chilling plate connected to the test tube rack, a fin type radiator disposed under the test tube rack, and a cooling fan disposed under the fin type radiator; wherein the raw reagent tubes are connected to the reaction tubes via a solenoid valve module and a peristaltic pump module, and the solenoid valve module and the peristaltic pump module are connected with a control device and are controlled by an upper computer control program.
-
公开(公告)号：US20190323092A1
公开(公告)日：2019-10-24
申请号：US16313906
申请日：2016-08-05
发明人： Ying YANG , Shengqi WANG , Zhe ZHOU , Min CHEN , Peng WANG , Zhen LI , Zongwei LI
IPC分类号： C12Q1/6895
摘要： A method for detecting trace fungi using single-cell sequencing and a fungi detection kit prepared by the method are disclosed. The method includes the steps of obtaining trace fungal cells, extracting fungal protoplasm by breaking the cell walls of fungi, extracting gDNA from trace fungal protoplasm and amplifying the gDNA, constructing trace gDNA library, genome sequencing, bioinformatics analysis and comparison, and determining the species of detected fungi, etc. The method realizes the high-efficiency detection of trace fungi, and can be directly applied to the isolation, detection, identification of trace and difficult-to-identify fungal samples or mixed samples, and in-depth study of genetic information. The fungi detection method and the kit are applicable to industrial production, environmental monitoring, air testing, soil testing, water quality testing, food testing, drug testing, cosmetics testing, health care products testing and medical testing, and other fields.
-
公开(公告)号：US20220090195A1
公开(公告)日：2022-03-24
申请号：US17419329
申请日：2019-03-12
发明人： Shengqi WANG , Qiqi LIU , Liyan LIU , Ying ZHANG , Yi ZHAO
IPC分类号： C12Q1/6876 , C12Q1/686 , C12Q1/6806
摘要： Disclosed by the present application are a structure of a double-stranded oligonucleotide nucleic acid probe, a method of use and applications thereof in nucleic acid fluorescence qualitative and quantitative analysis, medical diagnosis and life science researches. The double-stranded oligonucleotide nucleic acid probe is composed of two completely or partially base-complementary oligonucleotide strands; the end of each oligonucleotide strand may be connected to a fluorescent group or a corresponding fluorescent quenching group; and the two oligonucleotide probe strands may hybridize with a target nucleic acid sequence to be tested.
-
公开(公告)号：US20220072538A1
公开(公告)日：2022-03-10
申请号：US17278243
申请日：2018-12-28
发明人： Shengqi WANG , Rui XIAO , Zhen RONG
摘要： The present application relates to the field of biochip detection apparatus, and in particular to a fully automated biochip workstation and a detection method using same. Biochip detection apparatus comprises a cavity for carrying a test tube vial, the test tube vial being used for accommodating a sample to be detected and a biochip, the cavity having a temperature control function; a liquid storage device for storing liquid; a pumping device for pumping the liquid in the liquid storage device to the test tube vial, the pumping device being in fluid communication with a separation needle for injecting a mixed liquid into the test tube vial; an imaging device for monitoring the chip state in the test tube vial; and a data processing device configured to acquire hybridization results on the basis of information about a biochip state acquired by the imaging device. The biochip workstation provided in the present application implements the integration of biochip reactions and the output of detection results, improves the processing efficiency of samples, saves manpower, and has more stable and reliable results.
-
公开(公告)号：US20220221406A1
公开(公告)日：2022-07-14
申请号：US17614007
申请日：2019-08-14
发明人： Shengqi WANG , Rui XIAO , Zhen RONG
摘要： A sample rotating rack and a Raman spectrum detector. The sample rotating rack comprises a rotating body (1) and a plurality of sample carriers provided thereon. The plurality of sample carriers are distributed around the circumference of the rotating body (1) and can be irradiated by light rays located at the periphery of the rotating body (1). The sample rotating rack is provided with as many sample carriers as possible in a space as small as possible, and optical irradiation can be quickly conducted on a plurality of samples by rotating the rotating body (1). The Raman spectrum detector comprises a laser (6), a spectrum analyzer (7), a Raman probe (10), a rotating table (8) and a sample rotating rack; the sample rotating rack is arranged on the rotating table (8), the Raman probe (10) is arranged at the periphery of the sample rotating rack, and the Raman probe (10) is electrically connected to the laser (6) and the spectrum analyzer (7) respectively; and the laser (6) is used for emitting excitation light by means of the Raman probe (10), and the Raman probe (10) can receive Raman scattered light and return same to the spectrum analyzer (7). The Raman spectrum detector can rapidly perform optical analysis and test on a plurality of samples, and the operation is simple and the test efficiency is high.
-
公开(公告)号：US20220111379A1
公开(公告)日：2022-04-14
申请号：US17418947
申请日：2019-12-30
发明人： Shengqi WANG , Zhen RONG , Rui XIAO , Feng WANG
IPC分类号： B01L3/00
摘要： A reaction tube for multiple nucleic acid amplification, related to the applied technical field of biological science research and medical tests. The reaction tube for multiple nucleic acid amplification comprises a base (1) and multiple reaction tube cavities (3). The base (1) is provided with a reference plane (2). Openings of the multiple reaction cavities (3) are provided on the reference plane (2). The cavities are perpendicular to the reference plane (2) and extended towards the interior of the base (1). The reaction tube for multiple nucleic acid amplification provides the multiple reaction tube cavities (3) on the reference plane (2) of the base (1).
-
-
-
-
-
搜索结果
6 条记录 (耗时 0.013 秒)
