公开(公告)号：US20250102431A1

公开(公告)日：2025-03-27

申请号：US18972715

申请日：2024-12-06

Applicant: Access Medical Systems, Ltd.

Inventor： Pu Li ,  Indrani Chakraborty ,  Jessie Peh

IPC: G01N21/45 ,  G01N1/44 ,  G01N33/569

Abstract: The present invention is directed to a method of determining the percentage of full capsids in an adeno-associated virus (AAV) sample comprising AAVs. The invention features two basic steps. The first step involves separating each sample into two aliquots. One aliquot is kept at room temperature (RT), while the other aliquot is heated below the AAV melting point to disrupt the binding affinity of the AAVs to an anti-AAV coated probe. Heating an AAV aliquot leads to differential binding affinity of empty capsids versus full capsids on the anti-AAV-coated probe. The heated empty capsids retain their binding affinity to the probe, while the heated full capsids significantly reduce their binding affinity to the probe. The second step involves measuring the wavelength shifts of heated and RT aliquots due to light interference, and calculating their ratio. By quantitating the ratio against a standard curve, the amount of empty capsids versus full capsids in an unknown sample can be determined.

公开(公告)号：US20210041428A1

公开(公告)日：2021-02-11

申请号：US17069591

申请日：2020-10-13

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

公开(公告)号：US20180180606A1

公开(公告)日：2018-06-28

申请号：US15903838

申请日：2018-02-23

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53

CPC classification number: G01N33/54306 ,  G01N33/53 ,  G01N33/5306 ,  G01N33/533 ,  G01N33/543 ,  G01N33/582

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

公开(公告)号：US20250019780A1

公开(公告)日：2025-01-16

申请号：US18900261

申请日：2024-09-27

Applicant: Access Medical Systems, Ltd.

Inventor： Robert F. Zuk ,  Samuel Yang ,  Alex Ho Fai Lee ,  Indrani Chakraborty ,  Pu Li ,  Wai Choi

IPC: C12Q1/70 ,  G01N33/53 ,  G01N33/569 ,  G01N33/58

Abstract: The present invention is directed to a method of determining DNA concentration in a DNA virus. The invention features three basic steps. The initial step is the specific capture of a defined amount of virus capsid particles on a first solid phase. The second step is lysis of the capsid to release the virus DNA from the first solid phase into a lysis solution. After separating the lysis solution from the first solid phase, the third step is contacting the lysis solution with a second solid phase. The second solid phase captures total DNA derived from the captured capsid. The present invention is also directed to a method for measuring the percentage of full virus capsid, comprising first determining the ssDNA concentration in viruses, and then converting the ssDNA concentration to percentage of full virus capsid using a calibration curve having DNA concentration plotted against standards of % of full capsids.

公开(公告)号：US10809251B2

公开(公告)日：2020-10-20

申请号：US15903838

申请日：2018-02-23

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

公开(公告)号：US20240248084A1

公开(公告)日：2024-07-25

申请号：US18437032

申请日：2024-02-08

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Pu Li ,  Vasundhra Bahl ,  Samuel Yang

IPC: G01N33/557 ,  G01N33/543

CPC classification number: G01N33/557 ,  G01N33/54306

Abstract: The present invention is directed to biochemical assay methods, which uses a biotin-immobilized solid support surface for quantitating an analyte or measuring kinetic binding in different samples, from about 3 to 20 times, while maintaining acceptable assay performance. The methods use a streptavidin capture solution comprising streptavidin or preferably streptavidin polymers. The biotin-immobilized solid support surface is regenerated after each cycle of reactions by contacting the surface in an acidic solution having pH about 1-4, followed by DMSO. The regeneration step removes the bound immunocomplex and leaves the biotin on the surface.