公开(公告)号：US20180128826A1

公开(公告)日：2018-05-10

申请号：US15802075

申请日：2017-11-02

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Qing Xia

IPC: G01N33/543

CPC classification number: G01N33/54393 ,  G01N33/536 ,  G01N33/54386 ,  G01N2333/4737

Abstract: The present invention is directed an immunoassay method, which re-uses an antibody-immobilized test probe and reagents for quantitating an analyte in different samples, anywhere from about 3 to 20 times, while maintaining acceptable clinical assay performance. The method regenerates the test probe by dipping the test probe in an acidic solution having pH about 1-4, after the completion of each cycle of reaction. The present invention is also directed to a unitized cartridge (a strip) for an immunoassay test. Each unitized cartridge contains all necessary reagents can be used for 3-20 cycles to measure 3-20 different samples.

公开(公告)号：US20220018833A1

公开(公告)日：2022-01-20

申请号：US17488095

申请日：2021-09-28

Applicant: Access Medical Systems, Ltd.

Inventor： Robert F. Zuk ,  Qing Xia ,  Jenchieh Wu

IPC: G01N33/543 ,  G01N21/64

Abstract: The present invention is directed immunoassay methods, which re-use a hapten-immobilized test probe and reagents for quantitating an analyte in different samples, anywhere from about 3 to 20 times, while maintaining acceptable clinical assay performance. The methods use a dual antibody conjugate solution comprising an anti-hapten antibody and a capture antibody against the analyte in each cycle. After the completion of each cycle of reaction, the test probe is dipped in an acidic solution having pH about 1-4, to elute the immunocomplex formed on the probe and to regenerate the hapten-immobilized probe. The robustness of the hapten-coated solid phase allows utilization of multiple denaturation reagents for efficient elution of the immune complexes after each cycle without compromising the binding activity of the hapten on the solid phase.

公开(公告)号：US10809251B2

公开(公告)日：2020-10-20

申请号：US15903838

申请日：2018-02-23

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

公开(公告)号：US20210041428A1

公开(公告)日：2021-02-11

申请号：US17069591

申请日：2020-10-13

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

公开(公告)号：US20190250154A1

公开(公告)日：2019-08-15

申请号：US16394953

申请日：2019-04-25

Applicant: Access Medical Systems, Ltd.

Inventor： Robert F. Zuk ,  Qing Xia

IPC: G01N33/543

CPC classification number: G01N33/543

Abstract: The present invention is directed an immunoassay method using an interference detection system. The assay re-uses an antibody-immobilized test probe and reagents for quantitating an analyte in different samples, from about 3 to 20 times, while maintaining acceptable clinical assay performance. The method regenerates the test probe with an acidic solution after completion of each cycle of reactions. The present invention is also directed to a unitized cartridge (a strip) for an immunoassay test. Each unitized cartridge contains all necessary reagents and can be used for 3-20 cycles to measure 3-20 different samples.

公开(公告)号：US20180180606A1

公开(公告)日：2018-06-28

申请号：US15903838

申请日：2018-02-23

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53

CPC classification number: G01N33/54306 ,  G01N33/53 ,  G01N33/5306 ,  G01N33/533 ,  G01N33/543 ,  G01N33/582

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

公开(公告)号：US20200333337A1

公开(公告)日：2020-10-22

申请号：US16918911

申请日：2020-07-01

Applicant: Access Medical Systems, LTD.

Inventor： Robert F. Zuk ,  Qing Xia

IPC: G01N33/543 ,  G01N33/536

Abstract: The present invention is directed an immunoassay method, which re-uses an antibody-immobilized test probe and reagents for quantitating an analyte in different samples, anywhere from about 3 to 20 times, while maintaining acceptable clinical assay performance. The method regenerates the test probe by dipping the test probe in an acidic solution having pH about 1-4, after the completion of each cycle of reaction. The present invention is also directed to a unitized cartridge (a strip) for an immunoassay test. Each unitized cartridge contains all necessary reagents can be used for 3-20 cycles to measure 3-20 different samples.