公开(公告)号：US20240302386A1

公开(公告)日：2024-09-12

申请号：US18258993

申请日：2021-12-21

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Haode Chen ,  Heng Wu ,  Hong Tan ,  Robert F. Zuk

IPC: G01N33/74 ,  G01N21/64 ,  G01N21/76

CPC classification number: G01N33/74 ,  G01N21/6428 ,  G01N21/76 ,  G01N2021/6439 ,  G01N2333/585

Abstract: The present invention is directed to an immunoassay method for detecting an analyte in a liquid sample; and provides accuracy and reproducibility for both high concentration and low concentration samples. The method uses both fluorescence marker and chemiluminescence marker, and reads both fluorescence signal and chemiluminescence signal. The analyte concentration is determined based on either a calibration curve for the fluorescence signal, or a calibration curve chemiluminescence signal, using a pre-established analyte concentration value.

公开(公告)号：US20210041428A1

公开(公告)日：2021-02-11

申请号：US17069591

申请日：2020-10-13

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

公开(公告)号：US20180180606A1

公开(公告)日：2018-06-28

申请号：US15903838

申请日：2018-02-23

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53

CPC classification number: G01N33/54306 ,  G01N33/53 ,  G01N33/5306 ,  G01N33/533 ,  G01N33/543 ,  G01N33/582

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

公开(公告)号：US20240272153A1

公开(公告)日：2024-08-15

申请号：US18628497

申请日：2024-04-05

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Haode Chen ,  Robert F. Zuk

IPC: G01N33/543 ,  G01N21/64 ,  G01N33/533

CPC classification number: G01N33/54393 ,  G01N21/6428 ,  G01N33/533

Abstract: The present invention is directed biochemical assay methods with regenerated substrate surface for immunoassay reactions. The methods clean a ceramic or glass substrate surface with atmospheric gas plasma and then coat the cleaned surface directly (without further modification of the cleaned surface) with a hapten-polymer conjugate, a protein, or an anti-non-human IgG antibody as a first layer on the substrate surface for subsequence assay reactions. After the completion of each cycle of reactions, the substrate is cleaned by the same plasma cleaning procedure, to elute the organic molecules formed on the substrate surface and to regenerate the substrate for another cycle of immunoassay reactions. The regeneration protocols can be repeated 1-10 times and maintains acceptable assay performance.

公开(公告)号：US20240082836A1

公开(公告)日：2024-03-14

申请号：US18517999

申请日：2023-11-22

Applicant: Access Medical Systems, Ltd.

Inventor： Hong Tan ,  Haode Chen ,  Genqian Li

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/04 ,  B01L2300/0663 ,  B01L2300/0861 ,  B01L2400/043

Abstract: Introduced here are rotors that can be placed inside of microplate wells that include liquid samples. Each rotor can be comprised of a ferromagnetic material. Accordingly, when a rotor is subjected to an external rotational magnetic field, the rotor spins and agitates the liquid sample inside the corresponding well. The spin speed may be adjusted by changing the rotation speed, direction, and/or orientation of the external rotational magnetic field. The rotor typically includes a central cavity within which a probe can be suspended during the biochemical test.

公开(公告)号：US10809251B2

公开(公告)日：2020-10-20

申请号：US15903838

申请日：2018-02-23

Applicant: ACCESS MEDICAL SYSTEMS, LTD.

Inventor： Robert F. Zuk ,  Hong Tan ,  Qing Xia ,  Pu Li ,  Haode Chen ,  Heng Wu

IPC: G01N33/543 ,  G01N33/533 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.