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公开(公告)号:US20170159070A1
公开(公告)日:2017-06-08
申请号:US15316508
申请日:2014-06-06
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor: Chunxiao WU
CPC classification number: C12N15/85 , C12N9/1241 , C12N15/11 , C12N15/111 , C12N2303/00 , C12N2800/90 , C12N2830/48 , C12Y207/07
Abstract: Disclosed is a vector comprising a piggyBac like element (PLE) transposon and/or transposase. Also disclosed are vectors comprising a combination of the vectors of the present disclosure, a transformation system comprising the vectors of the present disclosure, an oligonucleotide primer, methods of amplifying nucleotide sequences, methods of transforming a cell with a gene and a transgenic organism.
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2.
公开(公告)号:US20170191034A1
公开(公告)日:2017-07-06
申请号:US15508897
申请日:2015-09-04
Inventor: Shu WANG , Chunxiao WU , Jieming ZENG , Yovita Ida PURWANTI , Andrew KHOO
IPC: C12N5/0784 , C12N5/095
CPC classification number: C12N5/0639 , A61K35/15 , A61K39/0011 , A61K2039/5154 , A61K2039/572 , C12N5/0695 , C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2502/30 , C12N2502/45 , C12N2506/30
Abstract: The invention concerns a method of preparing a composition comprising stimulated immune system cells such as dendritic cells (DC) for use in inducing immune response of cytotoxic T lymphocytes against colorectal cancer. The dendritic cells are pulsed by contact with colorectal cancer stem cells (CSC) or their fragments thereof. These colorectal CSCs are produced by OSKM (Oct4, Sox2, Klf4 and c-Myc) induced reprogramming of differentiated colorectal cancer cells (CRC) which results in undifferentiated colorectal CSC-like cells. Both the CSC-like cells and the lysates of heat-shocked CSC-like cells could be used as an accessible source of tumour antigens for DC pulsing to induce specific immune responses against colorectal CSCs.
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