公开(公告)号：US09581527B2

公开(公告)日：2017-02-28

申请号：US14246004

申请日：2014-04-04

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Kwong Joo Leck ,  Namyong Kim ,  Li Li ,  Yong Yeow Lee

IPC: B01L3/02 ,  G01N1/31 ,  B01L3/00 ,  G01N33/543 ,  C12M1/12 ,  G01N35/10

CPC classification number: G01N1/31 ,  B01L3/5088 ,  B01L2300/0609 ,  B01L2300/0829 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0415 ,  C12M25/01 ,  G01N33/54373 ,  G01N2035/1037

Abstract: A device for processing a sample in a liquid droplet containing a hydrophilic liquid is described. The device includes: a circumferential wall and a base including an immobilisation member. The circumferential wall and the base define a reservoir adapted to accommodate a hydrophobic medium immiscible with the liquid droplet. The medium is of a lower surface energy than a liquid of the liquid droplet. The immobilisation member includes a surface with a plurality of hydrophilic immobilisation areas and a hydrophobic area. The plurality of hydrophilic immobilisation areas is: (a) of a higher surface energy than the medium, (b) of a higher surface energy than the hydrophobic area, and (c) of a sufficient surface energy and a sufficient width to allow, in the medium, immobilisation of liquid droplets on the hydrophilic immobilisation areas via interfacial interactions. Methods of using and rinsing the device are also described.

Abstract translation: 描述了用于处理含有亲水性液体的液滴中的样品的装置。 该装置包括：周向壁和包括固定构件的底座。 圆周壁和基部限定适于容纳与液滴不混溶的疏水介质的储存器。 该介质具有比液滴的液体更低的表面能。 固定构件包括具有多个亲水固定区域和疏水区域的表面。 多个亲水固定区域是：（a）比介质具有更高的表面能，（b）具有比疏水区域更高的表面能，和（c）足够的表面能和足够的宽度，以允许 介质，通过界面相互作用将液滴固定在亲水固定区域上。 还描述了使用和冲洗该装置的方法。