公开(公告)号：US09480987B2

公开(公告)日：2016-11-01

申请号：US14048216

申请日：2013-10-08

申请人： Sasan Raghibizadeh ,  Charles Boone

发明人： Sasan Raghibizadeh ,  Charles Boone

IPC分类号： G06F7/00 ,  B01L9/00 ,  A61L2/24 ,  G01N35/00 ,  G01N35/10 ,  G01N35/04

CPC分类号： B01L9/52 ,  A61L2/24 ,  B01L9/543 ,  B01L9/547 ,  B01L2300/0819 ,  G01N35/0099 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/00326 ,  G01N2035/042 ,  G01N2035/1037

摘要： An apparatus and method for transferring plurality of samples from one sample container to another one is disclosed wherein each sample is randomly accessible and can be “cherry picked”. The disclosed method of actuation allows for using a smaller number of actuators than the number of sample transferring channels or pins and thereby simplifies the design and control of the sample transferring apparatus.

摘要翻译： 公开了一种用于将多个样品从一个样品容器转移到另一个样品的装置和方法，其中每个样品是随机存取的并且可以是“樱桃挑选”。 所公开的致动方法允许使用比样品传送通道或引脚的数量少的致动器，从而简化了样品转移装置的设计和控制。

公开(公告)号：US09182393B2

公开(公告)日：2015-11-10

申请号：US12132954

申请日：2008-06-04

申请人： Huan-Ping Wu

发明人： Huan-Ping Wu

IPC分类号： B01L3/00 ,  B01L9/00 ,  B05C1/00 ,  B05D5/00 ,  G01N33/543 ,  B01J19/00 ,  C40B60/14 ,  C40B40/00 ,  B01L3/02 ,  G01N35/10

CPC分类号： B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00443 ,  B01J2219/00509 ,  B01J2219/00603 ,  B01J2219/00605 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00725 ,  B01L3/0241 ,  B01L3/0248 ,  B01L3/0275 ,  B01L9/00 ,  B01L2300/0819 ,  B01L2400/025 ,  C40B60/14 ,  G01N33/54373 ,  G01N2035/1037

摘要： A microdeposition pin having a contact surface with at least one concave edge for creating microarrays and the like. The microdeposition pin may be used either alone or with a plurality of microdeposition pins in conjunction with a holder. The concave edge of the pin is especially adapted for helping to control the spreading of a deposited material. By selectively controlling the spread of the reagent composition from the microdeposition pin, the flow of the reagent composition from the deposition target area may be reduced. Sensor strips having raised substrate features with limited or no spreading of the reagent composition beyond the target area are disclosed.

摘要翻译： 具有与至少一个凹边缘的接触表面的微沉积销用于产生微阵列等。 微沉积销可以单独使用或与多个微沉积销一起用于保持器。 销的凹入边缘特别适于帮助控制沉积材料的铺展。 通过选择性地控制来自微沉积针的试剂组合物的扩散，可以减少来自沉积靶区域的试剂组合物的流动。 公开了具有凸起的衬底特征的传感器条，其中试剂组合物超出目标区域有限或不扩散。

公开(公告)号：US08845984B2

公开(公告)日：2014-09-30

申请号：US13615500

申请日：2012-09-13

申请人： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

发明人： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

IPC分类号： B01L9/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  G01N35/10

CPC分类号： B01L3/502715 ,  B01L3/50273 ,  B01L7/00 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  G01N2035/00306 ,  G01N2035/1037 ,  Y10T436/2575

摘要： The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

摘要翻译： 本发明提供了用于自动化生物处理的系统，装置，装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于：免疫沉淀，染色质免疫沉淀，重组蛋白分离，核酸分离和分离，蛋白标记，分离和分离，细胞分离和分离，食品安全分析和自动 珠基分离。 在一些实施方案中，本发明提供自动化系统，自动化装置，自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统，自动化装置，自动化盒和用于分离，制备和纯化核酸例如DNA或RNA或其片段的自动化方法，包括质粒DNA，基因组DNA，细菌DNA，病毒DNA和任何其它DNA， 以及用于自动化系统，自动化设备，自动化盒和用于处理，分离和纯化蛋白质，肽等的自动化盒和自动化方法。

公开(公告)号：US08691147B2

公开(公告)日：2014-04-08

申请号：US11984197

申请日：2007-11-14

申请人： Kwong Joo Leck ,  Nam Yong Kim ,  Jackie Y. Ying ,  Li Li ,  Yong Yeow Lee

发明人： Kwong Joo Leck ,  Nam Yong Kim ,  Jackie Y. Ying ,  Li Li ,  Yong Yeow Lee

IPC分类号： G01N21/00 ,  G01N1/00 ,  G01N33/00 ,  B01L9/00

CPC分类号： G01N1/31 ,  B01L3/5088 ,  B01L2300/0609 ,  B01L2300/0829 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0415 ,  C12M25/01 ,  G01N33/54373 ,  G01N2035/1037

摘要： The invention provides an apparatus and a method of processing a biological and/or chemical sample in a liquid droplet. The apparatus comprises a processing compartment, which is defined by a reservoir and an immobilization member. The processing compartment is further adapted to accommodate a medium, which is immiscible with the liquid droplet, and of a lower surface energy than the liquid of the liquid droplet. The reservoir is defined by a circumferential wall and a base. The immobilization member is arranged within the reservoir and comprises a surface that is patterned in such a way that it comprises at least one predefined immobilization area. The predefined immobilization area within the patterned surface is of a higher surface energy than the medium. The liquid droplet is disposed onto the predefined immobilization area, whereby the liquid droplet is immobilized thereon via hydrophilic-hydrophilic or hydrophobic-hydrophobic interactions.

摘要翻译： 本发明提供了一种在液滴中处理生物和/或化学样品的装置和方法。 该装置包括由储存器和固定构件限定的处理室。 处理室还适于容纳与液滴不混溶的介质和比液滴的液体低的表面能。 储存器由圆周壁和底座限定。 固定构件布置在储存器内并且包括以使得其包括至少一个预定义的固定区域的方式图案化的表面。 图案化表面内的预定义的固定区域具有比介质更高的表面能。 将液滴设置在预定义的固定区域上，由此通过亲水亲水或​​疏水 - 疏水相互作用将液滴固定在其上。

公开(公告)号：US08554362B2

公开(公告)日：2013-10-08

申请号：US13409620

申请日：2012-03-01

申请人： Sasan Raghibizadeh ,  Charles Boone

发明人： Sasan Raghibizadeh ,  Charles Boone

IPC分类号： G06F7/00

CPC分类号： B01L9/52 ,  A61L2/24 ,  B01L9/543 ,  B01L9/547 ,  B01L2300/0819 ,  G01N35/0099 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/00326 ,  G01N2035/042 ,  G01N2035/1037

摘要： An apparatus comprises a plurality of bushings arranged and held together in an array; and a plurality of pins residing in the plurality of bushings, wherein the plurality of pins are for transferring samples. Manufacturing tolerances for inside diameters of the bushings and outside diameters of the pins substantially match to allowing the pins to slide freely within the bushings. The plurality of bushings can reside in one or more plates, each of which having plurality of holes, with the plurality of bushings being held together in the array.

摘要翻译： 一种装置包括以阵列布置和保持在一起的多个衬套; 以及位于所述多个衬套中的多个销，其中所述多个销用于传送样品。 衬套的内径和销的外径的制造公差基本上匹配于允许销在衬套内自由滑动。 多个衬套可以驻留在一个或多个板中，每个板具有多个孔，多个衬套在阵列中保持在一起。

公开(公告)号：US08454908B2

公开(公告)日：2013-06-04

申请号：US13293050

申请日：2011-11-09

申请人： Daniel Lapen ,  David Zahniser ,  Mark Licari ,  Brian J. McKeen ,  Eric D. Yeaton ,  Dennis Poole

发明人： Daniel Lapen ,  David Zahniser ,  Mark Licari ,  Brian J. McKeen ,  Eric D. Yeaton ,  Dennis Poole

IPC分类号： A61B10/00

CPC分类号： G01N1/312 ,  G01N1/30 ,  G01N35/00029 ,  G01N35/0099 ,  G01N2035/00079 ,  G01N2035/00138 ,  G01N2035/00524 ,  G01N2035/1037

摘要： The systems and methods disclosed herein permit automated preparation of biological specimens for examination. The disclosed systems and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a biological specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation cycles for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining biological specimens.

公开(公告)号：US08414774B2

公开(公告)日：2013-04-09

申请号：US12560324

申请日：2009-09-15

申请人： William A. LaMarr ,  Can C. Ozbal ,  Arrin Katz ,  Donald Green

发明人： William A. LaMarr ,  Can C. Ozbal ,  Arrin Katz ,  Donald Green

IPC分类号： B01D15/08 ,  G01N30/02

CPC分类号： B01J19/0046 ,  B01D15/14 ,  B01D15/1871 ,  B01D15/305 ,  B01D15/34 ,  B01D15/361 ,  B01D2253/106 ,  B01D2253/112 ,  B01J2219/0036 ,  B01J2219/00387 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/00518 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/0072 ,  C40B60/14 ,  G01N30/24 ,  G01N30/40 ,  G01N30/466 ,  G01N30/7233 ,  G01N30/7266 ,  G01N30/84 ,  G01N35/00009 ,  G01N35/00584 ,  G01N35/08 ,  G01N35/1097 ,  G01N2030/324 ,  G01N2030/405 ,  G01N2030/626 ,  G01N2030/628 ,  G01N2030/8417 ,  G01N2035/1037 ,  G01N2035/1046 ,  Y10T436/25375 ,  Y10T436/2575 ,  G01N30/18

摘要： Aspects of the invention provide systems and methods for high-throughput screening of fluidic samples. In some embodiments, two chromatography columns are utilized in series. The first chromatography column can have a high affinity for phosphorylated compounds while the second chromatography column has a high affinity for one or more analytes of interest.

摘要翻译： 本发明的方面提供用于流体样品的高通量筛选的系统和方法。 在一些实施方案中，串联使用两个色谱柱。 第一色谱柱可以对磷酸化化合物具有高亲和力，而第二色谱柱对一种或多种感兴趣的分析物具有高亲和力。

公开(公告)号：US20130079247A1

公开(公告)日：2013-03-28

申请号：US13683214

申请日：2012-11-21

申请人： SEQUENOM, INC.

发明人： Xian-Wei YAO ,  Chao LIN ,  Paul HEANEY ,  Thomas BECKER ,  Aaron A. HANSON ,  Michael C. WILLIS

IPC分类号： G01N1/10

CPC分类号： B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/0244 ,  B01L3/0248 ,  B01L2200/0657 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N1/10 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/10 ,  G01N35/1016 ,  G01N35/1065 ,  G01N2035/00158 ,  G01N2035/1037 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/119163 ,  Y10T436/24 ,  Y10T436/2575

摘要： A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted.

摘要翻译： 提供了一种开槽针工具，包含销工具的输送系统，用于该系统的基板和使用该销工具和系统的方法。 开槽销工具包含多个销，其具有开槽端，设计成适合于沉积在诸如微阵列之类的表面上的每个材料位置，而不接触任何沉积材料。 通过将销工具与表面接触来传送样品; 当销的运动停止时，传递的量与销工具的接触表面的速度或液体的速度成比例。

公开(公告)号：US20130061880A1

公开(公告)日：2013-03-14

申请号：US13589671

申请日：2012-08-20

申请人： Peter HONKANEN ,  Albert BUKYS ,  Dave BRADBURY

发明人： Peter HONKANEN ,  Albert BUKYS ,  Dave BRADBURY

IPC分类号： B08B3/04

CPC分类号： B08B3/04 ,  B01L3/0244 ,  B01L99/00 ,  B41J29/17 ,  G01N35/1004 ,  G01N2035/1037

摘要： A multi-chambered deposition pin wash station is provided. The wash station includes a lower chamber and an upper drain basin connected by a plurality of wash tubes. Cleaning fluid is provided to the lower chamber and passes through the cleaning tubes into the upper drain basin. The cleaning tubes are adapted to clean a single deposition pin with a single tube per wash cycle.

摘要翻译： 提供了多腔沉积针清洗台。 洗涤站包括通过多个洗涤管连接的下室和上排水槽。 清洗液被提供到下室，并通过清洗管进入上排水槽。 清洗管适用于每个洗涤周期用单个管清洁单个沉积销。

公开(公告)号：US20130040376A1

公开(公告)日：2013-02-14

申请号：US13615500

申请日：2012-09-13

申请人： Joseph AMSHEY ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Tacher ,  Timothy Updyke ,  Kornelija Zgonc

发明人： Joseph AMSHEY ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Tacher ,  Timothy Updyke ,  Kornelija Zgonc

IPC分类号： C12M1/34 ,  C12M1/04 ,  C12M1/00

CPC分类号： B01L3/502715 ,  B01L3/50273 ,  B01L7/00 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  G01N2035/00306 ,  G01N2035/1037 ,  Y10T436/2575

摘要： The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

摘要翻译： 本发明提供了用于自动化生物处理的系统，装置，装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于：免疫沉淀，染色质免疫沉淀，重组蛋白分离，核酸分离和分离，蛋白标记，分离和分离，细胞分离和分离，食品安全分析和自动 珠基分离。 在一些实施方案中，本发明提供自动化系统，自动化装置，自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统，自动化装置，自动化盒和用于分离，制备和纯化核酸例如DNA或RNA或其片段的自动化方法，包括质粒DNA，基因组DNA，细菌DNA，病毒DNA和任何其它DNA， 以及用于自动化系统，自动化设备，自动化盒和用于处理，分离和纯化蛋白质，肽等的自动化盒和自动化方法。