公开(公告)号：US20200158642A1

公开(公告)日：2020-05-21

申请号：US16491960

申请日：2018-03-27

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Laura Sutarlie ,  Sian Yang Ow ,  Shilin Chen ,  Xiaodi Su

IPC: G01N21/64 ,  G01N33/542 ,  G01N33/569 ,  G01N15/06 ,  G01N21/359

Abstract: This invention relates to a sensor that detects bacteria cells comprising (a) a primary negatively charged, nanoparticulate sensing material; (b) a secondary positively charged, fluorescent sensing material; (c) a housing; and (d) at least one illuminator; wherein said housing contains said primary negatively charged, nanoparticulate sensing material, said secondary positively charged fluorescent sensing material and a sample potentially comprising bacteria in aqueous medium, wherein said illuminator provides light of at least one pre-specified wavelength λi to excite at least said secondary positively charged, fluorescent material, wherein said secondary positively charged, fluorescent material electrostatically attached to bacteria cells provides at least one fluorescent response at a second different wavelength λn wherein both i and n are integers, wherein said negatively charged, nanoparticulate sensing material electrostatically attached to said fluorescent material suppresses fluorescing of said fluorescent material at said second wavelength λn; and wherein said housing permits illumination of the contents of said housing by said illuminator and wherein said housing further permits the detection of a fluorescent response at a second wavelength λn. The negatively charged material includes (dsDNA coated) spherical AuNPs and graphene oxide (GO). The positively charged fluorescent material includes water soluble cationic conjugated polyelectrolytes (COPE) or positively charged peptide/polymer labeled with fluorescence dye. The sensor makes use of the FRET phenomenon between the primary and secondary sensing materials. The sensor allows making a distinction between living and dead bacteria and can measure the total bacteria count. A method for detecting bacteria utilizing the sensor is another part of the invention.

公开(公告)号：US20230075135A1

公开(公告)日：2023-03-09

申请号：US17798951

申请日：2021-02-11

Applicant: Agency for Science, Technology and Research

Inventor： Laura Sutarlie ,  Xiaodi Su ,  Yang Xu ,  Heng Li Chee ,  Sian Yang Ow ,  Takuya Sato

IPC: G01N33/569 ,  G01N33/543 ,  G01N33/58

Abstract: The present invention relates, in general terms, to nanoparticle solutions, kits, devices and methods of use thereof. The present invention is suitable for use in quantifying bacterial cells in a sample. The method of quantifying bacterial cells in a sample comprises passing the sample in a liquid form and an aqueous nanoparticle solution through a porous substrate such that the bacterial cells in the sample is trapped on the porous substrate and can be quantified by a colorimetric and/or fluorescence output emitted from the nanoparticle bound to the bacterial cells.

公开(公告)号：US20240369551A1

公开(公告)日：2024-11-07

申请号：US18284618

申请日：2022-03-29

Applicant: Agency for Science, Technology and Research

Inventor： Tedrick Thomas Salim Lew ,  Sian Yang Ow ,  Xiaodi Su ,  Laura Sutarlie ,  Khin Moh Moh Aung ,  Lisa Fong Poh Ng

IPC: G01N33/553 ,  G01N33/543 ,  G01N33/569

Abstract: The present invention relates to a nanosensor for detecting SARS-CoV-2 specific antibodies, wherein the nanosensor comprises a metal nanoparticle functionalised with one or more B-cell linear peptide epitopes derived from the spike (S) protein or nucleocapsid (N) protein of SARS-CoV-2. In some preferred embodiments, the metal nanoparticle comprises gold, silver, or a combination of gold and silver. The present invention also relates to various methods involving the use of the nanosensor.

公开(公告)号：US11371938B2

公开(公告)日：2022-06-28

申请号：US16491960

申请日：2018-03-27

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Laura Sutarlie ,  Sian Yang Ow ,  Shilin Chen ,  Xiaodi Su

IPC: G01N21/64 ,  G01N15/06 ,  G01N21/359 ,  G01N33/542 ,  G01N33/569 ,  B82Y15/00 ,  G01N15/00

Abstract: This disclosure relates to a sensor that detects bacteria cells comprising (a) a primary negatively charged, nanoparticulate sensing material; (b) a secondary positively charged, fluorescent sensing material; (c) a housing; and (d) at least one illuminator. This disclosure further relates to a method for detecting bacteria cells.