公开(公告)号：US20230075135A1

公开(公告)日：2023-03-09

申请号：US17798951

申请日：2021-02-11

Applicant: Agency for Science, Technology and Research

Inventor： Laura Sutarlie ,  Xiaodi Su ,  Yang Xu ,  Heng Li Chee ,  Sian Yang Ow ,  Takuya Sato

IPC: G01N33/569 ,  G01N33/543 ,  G01N33/58

Abstract: The present invention relates, in general terms, to nanoparticle solutions, kits, devices and methods of use thereof. The present invention is suitable for use in quantifying bacterial cells in a sample. The method of quantifying bacterial cells in a sample comprises passing the sample in a liquid form and an aqueous nanoparticle solution through a porous substrate such that the bacterial cells in the sample is trapped on the porous substrate and can be quantified by a colorimetric and/or fluorescence output emitted from the nanoparticle bound to the bacterial cells.

公开(公告)号：US20240369551A1

公开(公告)日：2024-11-07

申请号：US18284618

申请日：2022-03-29

Applicant: Agency for Science, Technology and Research

Inventor： Tedrick Thomas Salim Lew ,  Sian Yang Ow ,  Xiaodi Su ,  Laura Sutarlie ,  Khin Moh Moh Aung ,  Lisa Fong Poh Ng

IPC: G01N33/553 ,  G01N33/543 ,  G01N33/569

Abstract: The present invention relates to a nanosensor for detecting SARS-CoV-2 specific antibodies, wherein the nanosensor comprises a metal nanoparticle functionalised with one or more B-cell linear peptide epitopes derived from the spike (S) protein or nucleocapsid (N) protein of SARS-CoV-2. In some preferred embodiments, the metal nanoparticle comprises gold, silver, or a combination of gold and silver. The present invention also relates to various methods involving the use of the nanosensor.

公开(公告)号：US20200158642A1

公开(公告)日：2020-05-21

申请号：US16491960

申请日：2018-03-27

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Laura Sutarlie ,  Sian Yang Ow ,  Shilin Chen ,  Xiaodi Su

IPC: G01N21/64 ,  G01N33/542 ,  G01N33/569 ,  G01N15/06 ,  G01N21/359

Abstract: This invention relates to a sensor that detects bacteria cells comprising (a) a primary negatively charged, nanoparticulate sensing material; (b) a secondary positively charged, fluorescent sensing material; (c) a housing; and (d) at least one illuminator; wherein said housing contains said primary negatively charged, nanoparticulate sensing material, said secondary positively charged fluorescent sensing material and a sample potentially comprising bacteria in aqueous medium, wherein said illuminator provides light of at least one pre-specified wavelength λi to excite at least said secondary positively charged, fluorescent material, wherein said secondary positively charged, fluorescent material electrostatically attached to bacteria cells provides at least one fluorescent response at a second different wavelength λn wherein both i and n are integers, wherein said negatively charged, nanoparticulate sensing material electrostatically attached to said fluorescent material suppresses fluorescing of said fluorescent material at said second wavelength λn; and wherein said housing permits illumination of the contents of said housing by said illuminator and wherein said housing further permits the detection of a fluorescent response at a second wavelength λn. The negatively charged material includes (dsDNA coated) spherical AuNPs and graphene oxide (GO). The positively charged fluorescent material includes water soluble cationic conjugated polyelectrolytes (COPE) or positively charged peptide/polymer labeled with fluorescence dye. The sensor makes use of the FRET phenomenon between the primary and secondary sensing materials. The sensor allows making a distinction between living and dead bacteria and can measure the total bacteria count. A method for detecting bacteria utilizing the sensor is another part of the invention.

公开(公告)号：US20140134609A1

公开(公告)日：2014-05-15

申请号：US13968930

申请日：2013-08-16

Applicant: Agency for Science, Technology and Research

Inventor： Yen Nee Tan ,  Xiaodi Su

IPC: C12Q1/34

CPC classification number: G01N33/542 ,  C12Q1/6816 ,  G01N33/5308 ,  G01N33/54313 ,  C12Q2527/125 ,  C12Q2563/103 ,  C12Q2563/155

Abstract: The present invention provides a colorimetric method for detecting a polynucleotide strand binding molecule using one type of metal particles modified with a single type of interacting molecules. The interacting molecule is capable of specifically binding to nucleic and of protecting the metal particle from aggregation. Furthermore, the metal particles are capable of aggregation upon salt aggregation and/or cleavage of the interacting molecule, and colorimetric change changes upon aggregation.

Abstract translation: 本发明提供了使用一种类型的用单一相互作用分子修饰的金属颗粒检测多核苷酸链结合分子的比色法。 相互作用的分子能够特异性结合核酸并保护金属颗粒免受聚集。 此外，金属颗粒能够在相互作用的分子的盐聚集和/或裂解时聚集，并且在聚集时比色变化发生变化。

公开(公告)号：US20240337650A1

公开(公告)日：2024-10-10

申请号：US18574424

申请日：2022-06-27

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Xinting Zheng ,  Laura Sutarlie ,  Yong Yu ,  Xiaodi Su

IPC: G01N33/52 ,  G01N33/53 ,  G01N33/84

CPC classification number: G01N33/523 ,  G01N33/5308 ,  G01N33/84 ,  G01N2800/202

Abstract: There is provided herein a paper-based sensor for simultaneously determining a plurality of biomarkers present in a biological sample comprising a plurality of detection zones in fluid communication with a sampling zone, wherein said plurality of detection zones comprises sensing material specific to each of said plurality of biomarkers. There is also provided herein a method of manufacturing the paper-based sensor, a use of a paper-based sensor for wound diagnosis, a kit comprising the paper-based sensor and a method of diagnosing wound health.

公开(公告)号：US11371938B2

公开(公告)日：2022-06-28

申请号：US16491960

申请日：2018-03-27

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Laura Sutarlie ,  Sian Yang Ow ,  Shilin Chen ,  Xiaodi Su

IPC: G01N21/64 ,  G01N15/06 ,  G01N21/359 ,  G01N33/542 ,  G01N33/569 ,  B82Y15/00 ,  G01N15/00

Abstract: This disclosure relates to a sensor that detects bacteria cells comprising (a) a primary negatively charged, nanoparticulate sensing material; (b) a secondary positively charged, fluorescent sensing material; (c) a housing; and (d) at least one illuminator. This disclosure further relates to a method for detecting bacteria cells.

公开(公告)号：US09977014B2

公开(公告)日：2018-05-22

申请号：US14275526

申请日：2014-05-12

Applicant: Agency for Science, Technology and Research

Inventor： Steven Lukman ,  Siu Yee New ,  Xiaodi Su ,  Edwin Chong Wing Cheung

IPC: C12M1/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553

CPC classification number: G01N33/5308 ,  G01N33/54346 ,  G01N33/553

Abstract: The present invention provides a sensor for sensing nucleic acid-protein interactions, comprising a noble metal nanoparticle (NP), a double stranded nucleic acid molecule capable of binding with a protein in an aqueous solution and a fluorescent conjugated polymer (CP). The present invention also provides a method for sensing nucleic acid-protein interactions with the sensor as defined above.

公开(公告)号：US20140335510A1

公开(公告)日：2014-11-13

申请号：US14275526

申请日：2014-05-12

Applicant: Agency for Science, Technology and Research

Inventor： Steven Lukman ,  Siu Yee New ,  Xiaodi Su ,  Edwin Chong Wing Cheung

IPC: C12Q1/68

CPC classification number: G01N33/5308 ,  G01N33/54346 ,  G01N33/553

Abstract: The present invention provides a sensor for sensing nucleic acid-protein interactions, comprising a noble metal nanoparticle (NP), a double stranded nucleic acid molecule capable of binding with a protein in an aqueous solution and a fluorescent conjugated polymer (CP). The present invention also provides a method for sensing nucleic acid-protein interactions with the sensor as defined above.

Abstract translation: 本发明提供一种用于感测核酸 - 蛋白质相互作用的传感器，其包含贵金属纳米颗粒（NP），能够与水溶液中的蛋白质结合的双链核酸分子和荧光共轭聚合物（CP）。 本发明还提供了如上定义的用于感测与传感器的核酸 - 蛋白质相互作用的方法。