公开(公告)号：US09695216B2

公开(公告)日：2017-07-04

申请号：US14769245

申请日：2014-02-20

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon ,  Vincent Vagenende

IPC: C07K1/36 ,  B01D15/34 ,  B01D15/36 ,  C07K1/18 ,  C07K1/30 ,  C07K1/34 ,  B01D15/38

CPC classification number: C07K1/36 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/18 ,  C07K1/30 ,  C07K1/34

Abstract: A method includes (i) adding allantoin in a supersaturating amount to a protein preparation including a desired protein and at least one endotoxin as a contaminant, (ii) removing solids after the adding step to provide a sample for further purification by void exclusion chromatography on a packed particle bed of electropositive particles in a column, the packed particle bed having an interparticle volume, (iii) applying a sample volume to the packed particle bed, wherein the electropositive particles support void exclusion chromatography, and wherein the sample volume is not greater than the interparticle volume, and (iv) eluting a purified sample including the desired protein and a reduced amount of the endotoxin. The method is optionally carried out with only the allantoin treatment or only the void exclusion chromatography.