Abstract:
MYCELIA HAVING STRONG A-GALACTOSIDASE ACTIVITY AND VERY WEAK INVERTASE ACTIVITY ARE OBTAINED BY CULTURING A MOLD BELONGING TO THE GENUS ABSIDIA IN A CULTURE MEDIUM INCORPORATING THEREIN AT LEAST ONE MEMBER SELECTED FROM THE GROUP CONSISTING OF LACTOSE, MELIBIOSE, RAFFINOSE AND GALACTOSE. WHEN BEET MOLASSES IS TREATED WITH THE SAID MYCELIA, RAFFINOSE CONTAINED IN THE MOLASSES IS DECOMPOSED INTO SUCROSE AND GALACTOSE. THUS, THE YIELD OF SUCROSE CAN BE INCREASED.
Abstract:
In a method for producing sucrose from beets through a deionization treatment using ion-exchange resins, increasing the yield of sucrose by recovering sucrose from the molasses which is separated from sucrose when the massecuite resulting from the sugar-boiling process is subjected to centrifugal separation. Sucrose is recovered from the molasses by (i) allowing Alpha galactosidase to act upon the separated molasses thereby hydrolyzing the raffinose contained therein into sucrose and galactose and returning the resultant hydrolyzate to the purification process which includes the treatment of deionizing fresh diffusion juice with an ion-exchange resin, (ii) hydrolyzing the separated molasses into sucrose and raffinose with Alpha -galactosidase, deionizing the resultant hydrolyzate with an ion-exchange resins, and thereafter returning the deionized hydrolyzate to the purification process in which fresh diffusion juice is treated, or (iii) deionizing the separated molasses with an ion-exchange resins, subsequently hydrolyzing the raffinose contained therein into sucrose and galactose with Alpha -galactosidase, and thereafter returning the resulting hydrolyzate to the purification process in which fresh diffusion juice is treated; whereby the hydrolyzate is treated in the sugar-boiling process to effect recovery of sucrose contained therein. The molasses further separated centrifugally is treated again with Alpha -galactosidase and returned to the purification process in which fresh diffusion juice is treated.