公开(公告)号：US10144684B2

公开(公告)日：2018-12-04

申请号：US14867191

申请日：2015-09-28

Applicant: AJINOMOTO CO., INC.

Inventor： Hiroaki Rachi ,  Hiromoto Watanabe ,  Yosuke Nishio ,  Keiji Takita ,  Satoshi Hioki

IPC: C07C7/12 ,  C12P5/00 ,  C07C7/00 ,  C07C7/13 ,  B01D53/047

Abstract: Methods include contacting a fermented gas including isoprenoid compound with a porous adsorbent and desorbing isoprenoid compound adsorbed on the porous adsorbent. The fermented gas may be obtained by culturing a microorganism having an ability to produce isoprenoid compound.

公开(公告)号：US10184137B2

公开(公告)日：2019-01-22

申请号：US14670614

申请日：2015-03-27

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Mihara ,  Hiroaki Rachi ,  Yosuke Nishio ,  Yosifovna Joanna Katashkina ,  Dmitrievna Ekaterina Kazieva ,  Georgievna Irina Andreeva

IPC: C12P5/02 ,  C12N9/12 ,  C12P9/00 ,  C08F136/08 ,  B60C1/00 ,  C12P5/00 ,  C08L9/00

Abstract: A host cell includes a heterogeneous expression unit including: (a) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected from Methanocella, Corynebacterium, Methanosaeta, and Nitrosopumilus, and (b) a promoter operatively linked to the polynucleotide. The host cell is used to produce mevalonate kinase, mevalonate-5-phosphate, and isoprenoid compounds.

公开(公告)号：US20150275233A1

公开(公告)日：2015-10-01

申请号：US14670614

申请日：2015-03-27

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko MIHARA ,  Hiroaki Rachi ,  Yosuke Nishio ,  Yosifovna Joanna Katashkina ,  Dmitrievna Ekaterina Kazieva ,  Georgievna Irina Andreeva

IPC: C12P5/00 ,  C12P9/00 ,  C08F136/08 ,  C12N9/12

CPC classification number: C12P5/007 ,  B60C1/00 ,  C08F136/08 ,  C12N9/1205 ,  C12P9/00 ,  C12Y207/01036 ,  C08K5/0025 ,  C08L9/00

Abstract: A host cell includes a heterogeneous expression unit including: (a) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected from Methanocella, Corynebacterium, Methanosaeta, and Nitrosopumilus, and (b) a promoter operatively linked to the polynucleotide. The host cell is used to produce mevalonate kinase, mevalonate-5-phosphate, and isoprenoid compounds.

Abstract translation: 宿主细胞包括异源表达单位，其包括：（a）编码源自属于属于选自Methanocella，Corynebacterium，Methanosaeta和Nitrosopomilus的属的微生物的甲羟戊酸激酶的多核苷酸，和（b）与多核苷酸可操作连接的启动子。 宿主细胞用于产生甲羟戊酸激酶，甲羟戊酸-5-磷酸和类异戊二烯化合物。

公开(公告)号：US10144683B2

公开(公告)日：2018-12-04

申请号：US14867179

申请日：2015-09-28

Applicant: AJINOMOTO CO., INC.

Inventor： Hiroaki Rachi ,  Hiromoto Watanabe ,  Yosuke Nishio ,  Keiji Takita ,  Satoshi Hioki

IPC: C07C7/12 ,  C12P5/00 ,  C07C7/00 ,  C07C7/13 ,  B01D53/047

Abstract: Methods include contacting a fermented gas including isoprene with a porous adsorbent and desorbing isoprene adsorbed on the porous adsorbent. The fermented gas may be obtained by culturing a microorganism having an ability to produce isoprene.

公开(公告)号：US20170335349A1

公开(公告)日：2017-11-23

申请号：US15604772

申请日：2017-05-25

Applicant: Ajinomoto Co., Inc.

Inventor： Yoshinori TAJIMA ,  Hiroaki Rachi ,  Yosuke Nishio ,  Zhanna Iosifovna Katashkina ,  Valerievich Bylino Oleg

IPC: C12P5/00 ,  C08F36/08

CPC classification number: C12P5/007 ,  C08F36/08 ,  C12P5/00 ,  C12P5/026

Abstract: Producing an isoprenoid compound by: 1) culturing an isoprenoid compound-forming microorganism in the presence of a growth promoting agent at a sufficient concentration to grow the isoprenoid compound-forming microorganism; 2) decreasing a concentration of the growth promoting agent to induce formation of the isoprenoid compound by the isoprenoid compound-forming microorganism; and 3) culturing the isoprenoid compound-forming microorganism to form the isoprenoid compound, is characterized in that the growth phase of the isoprenoid compound-forming microorganism is separated from the formation phase of the isoprenoid compound.