公开(公告)号：US08962296B2

公开(公告)日：2015-02-24

申请号：US13832260

申请日：2013-03-15

Applicant: Bridgestone Corporation ,  Ajinomoto Co., Inc.

Inventor： Yasuyuki Hayashi ,  Minako Harada ,  Saaya Takaoka ,  Yasuo Fukushima ,  Keiichi Yokoyama ,  Yosuke Nishio ,  Yoshinori Tajima ,  Yoko Mihara ,  Kunio Nakata

IPC: C12N9/88 ,  C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C12N1/20 ,  C12N1/00 ,  C12P5/02 ,  C07H21/04 ,  C07K1/00 ,  C12P5/00

CPC classification number: C12P5/007 ,  C12N9/88 ,  C12P5/00 ,  C12Y402/03027

Abstract: The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c): (a) a polynucleotide comprising (i) the nucleotide sequence represented by SEQ ID NO:1, or (ii) the nucleotide sequence consisting of the nucleotide residues at positions 133 to 1785 in the nucleotide sequence represented by SEQ ID NO:1; (b) a polynucleotide that comprises a nucleotide sequence having 90% or more identity to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; or (c) a polynucleotide that hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; and the like.

Abstract translation: 本发明提供了用于建立优异的异戊二烯单体生产系统的方法。 具体而言，本发明提供下述（a），（b）或（c）所示的多核苷酸：（a）包含（i）由SEQ ID NO：1表示的核苷酸序列的多核苷酸，或（ii） 由SEQ ID NO：1所示的核苷酸序列的133〜1785位核苷酸残基组成的序列; （b）包含与上述（i）或（ii）的核苷酸序列具有90％以上同一性的核苷酸序列的多核苷酸，并编码具有异戊二烯合酶活性的蛋白质; 或（c）在严格条件下与由上述（i）或（ii）的核苷酸序列互补的核苷酸序列组成的多核苷酸杂交的多核苷酸，并编码具有异戊二烯合酶活性的蛋白质; 等等。

公开(公告)号：US20210071210A1

公开(公告)日：2021-03-11

申请号：US17036054

申请日：2020-09-29

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Mihara ,  Yohei Tatsukami ,  Yoshinori Tajima

IPC: C12P7/42 ,  C12P17/06

Abstract: The present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, productivity of which has been enhanced. Specifically, the present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, by producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein such as the following: (A) a protein having an amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14; (B) a protein having an amino acid sequence containing one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14, and having a linoleate 13-hydratase activity; and (C) a protein having an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13 or 14, and having a linoleate 13-hydratase activity.

公开(公告)号：US11499171B2

公开(公告)日：2022-11-15

申请号：US17036054

申请日：2020-09-29

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Mihara ,  Yohei Tatsukami ,  Yoshinori Tajima

IPC: C12P7/42 ,  C12N9/88 ,  C12P17/06

Abstract: The present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, productivity of which has been enhanced. Specifically, the present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, by producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein such as the following: (A) a protein having an amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14; (B) a protein having an amino acid sequence containing one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14, and having a linoleate 13-hydratase activity; and (C) a protein having an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13 or 14, and having a linoleate 13-hydratase activity.

公开(公告)号：US10184137B2

公开(公告)日：2019-01-22

申请号：US14670614

申请日：2015-03-27

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko Mihara ,  Hiroaki Rachi ,  Yosuke Nishio ,  Yosifovna Joanna Katashkina ,  Dmitrievna Ekaterina Kazieva ,  Georgievna Irina Andreeva

IPC: C12P5/02 ,  C12N9/12 ,  C12P9/00 ,  C08F136/08 ,  B60C1/00 ,  C12P5/00 ,  C08L9/00

Abstract: A host cell includes a heterogeneous expression unit including: (a) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected from Methanocella, Corynebacterium, Methanosaeta, and Nitrosopumilus, and (b) a promoter operatively linked to the polynucleotide. The host cell is used to produce mevalonate kinase, mevalonate-5-phosphate, and isoprenoid compounds.