公开(公告)号：US20220396819A1

公开(公告)日：2022-12-15

申请号：US17831877

申请日：2022-06-03

Applicant: AJINOMOTO CO., INC.

Inventor： Kenichiro Ito ,  Yoshihiko Matsuda ,  Naoko Yamada ,  Yoshimi Kikuchi ,  Atsushi Konishi ,  Michiya Kanzaki ,  Kazutoshi Takahashi ,  Hiroki Yamaguchi ,  Ayako Fujiwara ,  Kyohei Miyairi

IPC: C12P21/02

Abstract: Provided are a method for producing a complicated peptide molecule, such as a dimeric peptide, in a simpler manner at lower cost compared with the conventional chemical synthesis methods; and a dimeric peptide which comprises a specific short linker and has a satisfactory activity; and others.

公开(公告)号：US20240026343A1

公开(公告)日：2024-01-25

申请号：US18356516

申请日：2023-07-21

Applicant: AJINOMOTO CO., INC.

Inventor： Kenichiro Ito ,  Kyohei Miyairi

IPC: C12N15/10

CPC classification number: C12N15/1034

Abstract: Provided is a quick and simple method for screening dimerized cyclic peptides, a library therefor, and molecules including cyclic peptides that can be used in the method and the library. For example, the method for screening dimerized cyclic peptides includes (1) a step in which, in a cell-free expression system, a cyclic peptide, a dimerization domain including a cysteine residue and a leucine zipper region, and at least one polypeptide including a peptide linker existing between the cyclic peptide and the dimerization domain are synthesized, and a dimer of the polypeptide is prepared, and (2) a step in which activity based on binding between the dimer and a target molecule is measured.