公开(公告)号：US20140220637A1

公开(公告)日：2014-08-07

申请号：US14250548

申请日：2014-04-11

Applicant: AJINOMOTO CO., INC.

Inventor： Noriko Tsurui ,  Hiroshi Itaya ,  Yoshimi Kikuchi

IPC: C12P21/02

CPC classification number: C12P21/02 ,  C07K14/34 ,  C07K2319/02 ,  C07K2319/50 ,  C12N15/77

Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium is cultured so that it secretes a heterologous protein, the bacterium having a genetic construct which includes a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, which is ligated downstream from the promoter sequence, and a nucleic acid sequence coding for a fusion protein having an amino acid sequence that includes Gln-Glu-Thr and the heterologous protein, which is ligated downstream from the nucleic acid sequence coding for the signal peptide.

Abstract translation: 描述了通过棒状细菌改善异源蛋白质的分泌生产的新技术，从而提供了用于分泌异源蛋白质的方法。 培养棒状细菌以分泌异源蛋白质，该细菌具有遗传构建体，其包含在棒状细菌中起作用的启动子序列，编码在棒状细菌中起作用的信号肽的核酸序列，其被连接 以及编码具有包含Gln-Glu-Thr和异源蛋白质的氨基酸序列的融合蛋白的核酸序列，其连接于编码信号肽的核酸序列的下游。

公开(公告)号：US09376701B2

公开(公告)日：2016-06-28

申请号：US14250548

申请日：2014-04-11

Applicant: AJINOMOTO CO., INC.

Inventor： Noriko Tsurui ,  Hiroshi Itaya ,  Yoshimi Kikuchi

IPC: C12P21/02 ,  C07K14/34 ,  C12N15/77

CPC classification number: C12P21/02 ,  C07K14/34 ,  C07K2319/02 ,  C07K2319/50 ,  C12N15/77

Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium is cultured so that it secretes a heterologous protein, the bacterium having a genetic construct which includes a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, which is ligated downstream from the promoter sequence, and a nucleic acid sequence coding for a fusion protein having an amino acid sequence that includes Gln-Glu-Thr and the heterologous protein, which is ligated downstream from the nucleic acid sequence coding for the signal peptide.

Abstract translation: 描述了通过棒状细菌改善异源蛋白质的分泌生产的新技术，从而提供了用于分泌异源蛋白质的方法。 培养棒状细菌以分泌异源蛋白质，该细菌具有遗传构建体，其包含在棒状细菌中起作用的启动子序列，编码在棒状细菌中起作用的信号肽的核酸序列，其被连接 以及编码具有包含Gln-Glu-Thr和异源蛋白质的氨基酸序列的融合蛋白的核酸序列，其连接于编码信号肽的核酸序列的下游。

公开(公告)号：US20220396819A1

公开(公告)日：2022-12-15

申请号：US17831877

申请日：2022-06-03

Applicant: AJINOMOTO CO., INC.

Inventor： Kenichiro Ito ,  Yoshihiko Matsuda ,  Naoko Yamada ,  Yoshimi Kikuchi ,  Atsushi Konishi ,  Michiya Kanzaki ,  Kazutoshi Takahashi ,  Hiroki Yamaguchi ,  Ayako Fujiwara ,  Kyohei Miyairi

IPC: C12P21/02

Abstract: Provided are a method for producing a complicated peptide molecule, such as a dimeric peptide, in a simpler manner at lower cost compared with the conventional chemical synthesis methods; and a dimeric peptide which comprises a specific short linker and has a satisfactory activity; and others.

公开(公告)号：US20140255996A1

公开(公告)日：2014-09-11

申请号：US14264353

申请日：2014-04-29

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Matsuda ,  Hiroshi Itaya ,  Yoshimi Kikuchi ,  Haruki Beppu ,  Jurgis Antanas Vladovich Jomantas ,  Ekaterina Aleksandrovna Kutukova

IPC: C07K16/00

CPC classification number: C07K16/00 ,  C07K14/34 ,  C12N15/77

Abstract: A method for secretory production of a heterologous protein is provided by developing a novel technique for improving ability of a coryneform bacterium to produce a heterologous protein by secretory production. By utilizing a coryneform bacterium having an ability to produce a heterologous protein by secretory production which has been modified so that the activity of a penicillin-binding protein is reduced and in which the activity of a cell surface layer protein has been reduced as an expression host, a heterologous protein is produced by secretory production.

Abstract translation: 通过开发用于通过分泌生产改进棒状细菌以产生异源蛋白质的能力的新技术来提供异源蛋白质的分泌生产的方法。 通过使用具有通过分泌生产产生异源蛋白的能力的棒状细菌，其已经被修饰，使得青霉素结合蛋白的活性降低，并且细胞表面蛋白的活性已经被降低为表达宿主 通过分泌生产产生异源蛋白质。

公开(公告)号：US09187554B2

公开(公告)日：2015-11-17

申请号：US14264353

申请日：2014-04-29

Applicant: AJINOMOTO CO., INC.

Inventor： Yoshihiko Matsuda ,  Hiroshi Itaya ,  Yoshimi Kikuchi ,  Haruki Beppu ,  Jurgis Antanas Vladovich Jomantas ,  Ekaterina Aleksandrovna Kutukova

IPC: C07K16/00 ,  C07K16/34 ,  C12N15/77 ,  C07K14/34

CPC classification number: C07K16/00 ,  C07K14/34 ,  C12N15/77

Abstract: A method for secretory production of a heterologous protein is provided by developing a novel technique for improving ability of a coryneform bacterium to produce a heterologous protein by secretory production. By utilizing a coryneform bacterium having an ability to produce a heterologous protein by secretory production which has been modified so that the activity of a penicillin-binding protein is reduced and in which the activity of a cell surface layer protein has been reduced as an expression host, a heterologous protein is produced by secretory production.

Abstract translation: 通过开发用于通过分泌生产改进棒状细菌以产生异源蛋白质的能力的新技术来提供异源蛋白质的分泌生产的方法。 通过使用具有通过分泌生产产生异源蛋白的能力的棒状细菌，其已经被修饰，使得青霉素结合蛋白的活性降低，并且细胞表面蛋白的活性已经被降低为表达宿主 通过分泌生产产生异源蛋白质。