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1.发明申请公开(公告)号：US20220396819A1
公开(公告)日：2022-12-15
申请号：US17831877
申请日：2022-06-03
Applicant: AJINOMOTO CO., INC.
Inventor： Kenichiro Ito , Yoshihiko Matsuda , Naoko Yamada , Yoshimi Kikuchi , Atsushi Konishi , Michiya Kanzaki , Kazutoshi Takahashi , Hiroki Yamaguchi , Ayako Fujiwara , Kyohei Miyairi
IPC: C12P21/02
Abstract: Provided are a method for producing a complicated peptide molecule, such as a dimeric peptide, in a simpler manner at lower cost compared with the conventional chemical synthesis methods; and a dimeric peptide which comprises a specific short linker and has a satisfactory activity; and others.
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公开(公告)号：US20190185904A1
公开(公告)日：2019-06-20
申请号：US16293794
申请日：2019-03-06
Applicant: AJINOMOTO CO., INC.
Inventor： Hiroki Yamaguchi , Kazutoshi Takahashi , Moemi Tatsumi
IPC: C12Q1/26
CPC classification number: C12Q1/26 , C12M1/34 , C12N15/09 , C12P21/02 , C12Q1/00 , C12Y113/11011 , G01N27/416 , G01N33/68
Abstract: A mutated tryptophan oxidase suitable for practical implementation is described herein. Specifically, a mutated tryptophan oxidase wherein at least one amino acid residue of a wild-type tryptophan oxidase is mutated and, as a result, has higher tryptophan oxidase activity and/or stability as compared to the wild-type tryptophan oxidase. The mutated tryptophan oxidase can be derived from a wild-type tryptophan oxidase having at least one of Motifs (2), (3), (5), (7), (9), (11), (13), and (14), and at least one amino acid residue in any of these motifs can have mutation. The mutated tryptophan oxidase also can have a mutation of one or more amino acid residues in an amino acid sequence represented by SEQ ID NO: 2 and a sequence homologous thereto.
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Patent Agency Ranking
公开(公告)号：US20230203456A1
公开(公告)日：2023-06-29
申请号：US17947596
申请日：2022-09-19
Applicant: AJINOMOTO CO., INC.
Inventor： Kazutoshi Takahashi , Hiroki Yamaguchi , Uno Tagami
CPC classification number: C12N9/0022 , C12Y104/03011 , C12Q1/005 , C12P7/50
Abstract: The present invention provides an alternative L-glutamate oxidase that allows for measurement of L-glutamate. More specifically, the present invention provides the following L-glutamate oxidase mutant (a) or (b) and the like: (a) an L-glutamate oxidase mutant including an amino acid sequence that has 90% or more identity to an amino acid sequence of SEQ ID NO: 3 and exhibits an activity of oxidizing L-glutamate, except an L-glutamate oxidase including an amino acid sequence of SEQ ID NO: 1; or (b) an L-glutamate oxidase mutant comprising a peptide linker consisting of 1 to 20 amino acid residues which is inserted into one or more sites selected from the group consisting of (1) a site in a region proximity to a boundary between α1 and α2 regions, (2) a site in a region proximity to a boundary between α2 and γ regions and (3) a site in a region proximity to a boundary between γ and β regions in the L-glutamate oxidase mutant (a), and having the activity of oxidizing L-glutamate.
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公开(公告)号：US20160002610A1
公开(公告)日：2016-01-07
申请号：US14862323
申请日：2015-09-23
Applicant: AJINOMOTO CO., INC.
Inventor： Yuya Kodama , Wataru Hoshino , Kazutoshi Takahashi , Moemi Ito
IPC: C12N9/06 , G01N27/327 , C12Q1/00 , C12Q1/26 , C12P7/40
CPC classification number: C12N9/0022 , C12P7/40 , C12P7/42 , C12Q1/005 , C12Q1/26 , C12Y104/03019 , G01N27/3271 , G01N2333/90638
Abstract: The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.
Abstract translation: 本发明提供了一种新的酶和使用酶测定甘氨酸浓度的方法。 具体地说，本发明提供一种其中至少一个氨基酸残基被突变以改善与甘氨酸测量相关的甘氨酸氧化酶的性质的酶（例如甘氨酸的甘氨酸氧化酶的活性，甘氨酸的热稳定性 氧化酶和甘氨酸氧化酶对甘氨酸的底物特异性）; 以及分析甘氨酸的方法，其包括使用所述修饰酶测定测试样品中所含的甘氨酸; 等等。
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公开(公告)号：US20220235333A1
公开(公告)日：2022-07-28
申请号：US17714537
申请日：2022-04-06
Applicant: AJINOMOTO CO., INC.
Inventor： Moemi Tatsumi , Kazutoshi Takahashi , Uno Tagami , Hiroki Yamaguchi
Abstract: The present invention provides a unit and a method useful for more precise phenylalanine measurement. More specifically, the present invention provides a modified phenylalanine dehydrogenase that includes a mutation of at least one amino acid residue so as to improve the characteristics (for example, substrate specificity, solubility, and phenylalanine dehydrogenase activity) of a phenylalanine dehydrogenase related to measurement of phenylalanine, a method for analyzing phenylalanine by measuring phenylalanine contained in a test sample using the modified phenylalanine dehydrogenase, and others.
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公开(公告)号：US10961560B2
公开(公告)日：2021-03-30
申请号：US16293794
申请日：2019-03-06
Applicant: AJINOMOTO CO., INC.
Inventor： Hiroki Yamaguchi , Kazutoshi Takahashi , Moemi Tatsumi
Abstract: A mutated tryptophan oxidase suitable for practical implementation is described herein. Specifically, a mutated tryptophan oxidase wherein at least one amino acid residue of a wild-type tryptophan oxidase is mutated and, as a result, has higher tryptophan oxidase activity and/or stability as compared to the wild-type tryptophan oxidase. The mutated tryptophan oxidase can be derived from a wild-type tryptophan oxidase having at least one of Motifs (2), (3), (5), (7), (9), (11), (13), and (14), and at least one amino acid residue in any of these motifs can have mutation. The mutated tryptophan oxidase also can have a mutation of one or more amino acid residues in an amino acid sequence represented by SEQ ID NO: 2 and a sequence homologous thereto.
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公开(公告)号：US09976126B2
公开(公告)日：2018-05-22
申请号：US14862323
申请日：2015-09-23
Applicant: AJINOMOTO CO., INC.
Inventor： Yuya Kodama , Wataru Hoshino , Kazutoshi Takahashi , Moemi Ito
CPC classification number: C12N9/0022 , C12P7/40 , C12P7/42 , C12Q1/005 , C12Q1/26 , C12Y104/03019 , G01N27/3271 , G01N2333/90638
Abstract: The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.
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