公开(公告)号：US11046986B2

公开(公告)日：2021-06-29

申请号：US16582385

申请日：2019-09-25

Applicant: AJINOMOTO CO., INC.

Inventor： Shuhei Hashiro ,  Hisashi Yasueda ,  Mayu Mitsuhashi ,  Sergei Vladimirovich Mashko ,  Aleksandr Aleksandrovich Krylov ,  Yuliya Sergeevna Lobanova

IPC: C12P19/34 ,  C12N15/10 ,  C12N15/77 ,  C12R1/13 ,  C12R1/15

Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.

公开(公告)号：US11884951B2

公开(公告)日：2024-01-30

申请号：US17326677

申请日：2021-05-21

Applicant: AJINOMOTO CO., INC.

Inventor： Shuhei Hashiro ,  Hisashi Yasueda ,  Mayu Mitsuhashi ,  Sergei Vladimirovich Mashko ,  Aleksandr Aleksandrovich Krylov ,  Yuliya Sergeevna Lobanova

IPC: C12P19/34 ,  C12N15/10 ,  C12N15/77 ,  C12N1/20 ,  C12R1/13 ,  C12R1/05

CPC classification number: C12P19/34 ,  C12N1/205 ,  C12N15/1003 ,  C12N15/77 ,  C12R2001/05 ,  C12R2001/13

Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.

公开(公告)号：US20210269840A1

公开(公告)日：2021-09-02

申请号：US17326677

申请日：2021-05-21

Applicant: AJINOMOTO CO., INC.

Inventor： Shuhei Hashiro ,  Hisashi Yasueda ,  Mayu Mitsuhashi ,  Sergei Vladimirovich Mashko ,  Aleksandr Aleksandrovich Krylov ,  Yuliya Sergeevna Lobanova

IPC: C12P19/34 ,  C12N15/10 ,  C12N15/77 ,  C12N1/20

Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.

公开(公告)号：US20200024629A1

公开(公告)日：2020-01-23

申请号：US16582385

申请日：2019-09-25

Applicant: AJINOMOTO CO., INC.

Inventor： Shuhei Hashiro ,  Hisashi Yasueda ,  Mayu Mitsuhashi ,  Sergei Vladimirovich Mashko ,  Aleksandr Aleksandrovich Krylov ,  Yuliya Sergeevna Lobanova

IPC: C12P19/34 ,  C12N15/77 ,  C12R1/13 ,  C12N15/10

Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.