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公开(公告)号:US11046986B2
公开(公告)日:2021-06-29
申请号:US16582385
申请日:2019-09-25
Applicant: AJINOMOTO CO., INC.
Inventor: Shuhei Hashiro , Hisashi Yasueda , Mayu Mitsuhashi , Sergei Vladimirovich Mashko , Aleksandr Aleksandrovich Krylov , Yuliya Sergeevna Lobanova
Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
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公开(公告)号:US11884951B2
公开(公告)日:2024-01-30
申请号:US17326677
申请日:2021-05-21
Applicant: AJINOMOTO CO., INC.
Inventor: Shuhei Hashiro , Hisashi Yasueda , Mayu Mitsuhashi , Sergei Vladimirovich Mashko , Aleksandr Aleksandrovich Krylov , Yuliya Sergeevna Lobanova
CPC classification number: C12P19/34 , C12N1/205 , C12N15/1003 , C12N15/77 , C12R2001/05 , C12R2001/13
Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
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公开(公告)号:US10941390B2
公开(公告)日:2021-03-09
申请号:US16897482
申请日:2020-06-10
Applicant: AJINOMOTO CO., INC.
Inventor: Noriko Yokoyama , Mayu Nakano , Shuhei Hashiro , Masako Hiraga , Kazutaka Shimbo , Ayaka Tokunaga
IPC: C12N9/82 , A23L27/60 , A23L13/00 , A23C13/16 , C12P21/00 , A21D2/26 , A23C9/12 , A23L13/40 , A23L11/00
Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.
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公开(公告)号:US20130084609A1
公开(公告)日:2013-04-04
申请号:US13707950
申请日:2012-12-07
Applicant: AJINOMOTO CO., INC.
Inventor: Shigeo Suzuki , Yoshihiro Usuda , Shuhei Hashiro
IPC: C12P13/04
Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.
Abstract translation: 通过在含有促进L-氨基酸的生成和积累的微藻的加工物的培养基中培养具有L-氨基酸生产能力的细菌来生产L-氨基酸。 通过破坏微藻的培养和/或提取微藻的培养物,或分馏微藻的培养物或破坏的培养物来生产该过程产物。 经加工的产品含有由微藻生产的有机物质,破碎的微藻培养物的水解产物和/或微藻培养物的提取物或分馏产物的混合物。 经加工的产品还可以含有淀粉或脂肪和油的水解产物的糖化产物。 培养细菌以在培养物中产生和积累L-氨基酸,并从培养物中收集L-氨基酸。
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公开(公告)号:US20210269840A1
公开(公告)日:2021-09-02
申请号:US17326677
申请日:2021-05-21
Applicant: AJINOMOTO CO., INC.
Inventor: Shuhei Hashiro , Hisashi Yasueda , Mayu Mitsuhashi , Sergei Vladimirovich Mashko , Aleksandr Aleksandrovich Krylov , Yuliya Sergeevna Lobanova
Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
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公开(公告)号:US20210024931A1
公开(公告)日:2021-01-28
申请号:US16993692
申请日:2020-08-14
Applicant: AJINOMOTO CO., INC
Inventor: Shuhei Hashiro , Hisashi Yasueda , Mayu Nakano , Teruyuki Niimi , Haruka Kawaguchi
IPC: C12N15/113 , C12N9/22 , C12N15/70 , C12N15/77 , A01N63/10
Abstract: A method for efficiently inducing RNA silencing in a target organism is provided. RNA silencing is induced in a target organism by allowing the target organism to ingest cells of a microorganism, wherein the microorganism is able to produce RNA that induces RNA silencing after treating the cells with an organic solvent.
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公开(公告)号:US20200318093A1
公开(公告)日:2020-10-08
申请号:US16897482
申请日:2020-06-10
Applicant: AJINOMOTO CO., INC.
Inventor: Noriko YOKOYAMA , Mayu Nakano , Shuhei Hashiro , Masako Hiraga , Kazutaka Shimbo , Ayaka Tokunaga
Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.
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公开(公告)号:US10717971B2
公开(公告)日:2020-07-21
申请号:US15252664
申请日:2016-08-31
Applicant: AJINOMOTO CO., INC.
Inventor: Noriko Yokoyama , Mayu Nakano , Shuhei Hashiro , Masako Hiraga , Kazutaka Shimbo , Ayaka Tokunaga
IPC: C12N9/82 , C12N15/52 , C12N9/78 , A23C13/16 , C12P21/00 , A21D2/26 , A23C9/12 , A23L27/60 , A23L13/40 , A23L11/00
Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.
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公开(公告)号:US20200024629A1
公开(公告)日:2020-01-23
申请号:US16582385
申请日:2019-09-25
Applicant: AJINOMOTO CO., INC.
Inventor: Shuhei Hashiro , Hisashi Yasueda , Mayu Mitsuhashi , Sergei Vladimirovich Mashko , Aleksandr Aleksandrovich Krylov , Yuliya Sergeevna Lobanova
Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
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公开(公告)号:US08728772B2
公开(公告)日:2014-05-20
申请号:US13707950
申请日:2012-12-07
Applicant: Ajinomoto Co., Inc.
Inventor: Shigeo Suzuki , Yoshihiro Usuda , Shuhei Hashiro
Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.
Abstract translation: 通过在含有促进L-氨基酸的生成和积累的微藻的加工物的培养基中培养具有L-氨基酸生产能力的细菌来生产L-氨基酸。 通过破坏微藻的培养和/或提取微藻的培养物,或分馏微藻的培养物或破坏的培养物来生产该过程产物。 经加工的产品含有由微藻生产的有机物质,破碎的微藻培养物的水解产物和/或微藻培养物的提取物或分馏产物的混合物。 经加工的产品还可以含有淀粉或脂肪和油的水解产物的糖化产物。 培养细菌以在培养物中产生和积累L-氨基酸,并从培养物中收集L-氨基酸。
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