公开(公告)号：US11965198B2

公开(公告)日：2024-04-23

申请号：US17221308

申请日：2021-04-02

申请人： AJINOMOTO CO., INC.

发明人： Ekaterina Andrianova ,  Maria Kharchenko ,  Natalia Zakataeva ,  Elvira Voroshilova ,  Aleksander Krylov ,  Evgeniya Malykh ,  Sergei Mashko ,  Natalia Stoynova ,  Mikhail Baboshin ,  Lyubov Golubeva ,  Ekaterina Kovaleva ,  Mikhail Shupletsov

IPC分类号： C12P21/02 ,  C12N1/20 ,  C12N9/14 ,  C12N15/52 ,  C12P5/00 ,  C12P19/02 ,  C12R1/15 ,  C12R1/19

CPC分类号： C12P21/02 ,  C12N1/20 ,  C12N9/14 ,  C12N15/52 ,  C12P5/007 ,  C12P19/02 ,  C12R2001/15 ,  C12R2001/19 ,  C12Y306/01009

摘要： The present invention provides a method for producing a target substance, the biosynthetic pathway of which is ATP-dependent, for example, amino acids, nucleosides, nucleotides, isoprenoids, and peptides, by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having H+-translocating membrane-bound pyrophosphatase activity, for example, the hppA gene native to R. rubrum or a variant thereof.

公开(公告)号：US20210269842A1

公开(公告)日：2021-09-02

申请号：US17221308

申请日：2021-04-02

申请人： AJINOMOTO CO., INC.

发明人： Ekaterina Andrianova ,  Maria Kharchenko ,  Natalia Zakataeva ,  Elvira Voroshilova ,  Aleksander Krylov ,  Evgeniya Malykh ,  Sergei Mashko ,  Natalia Stoynova ,  Mikhail Baboshin ,  Lyubov Golubeva ,  Ekaterina Kovaleva ,  Mikhail Shupletsov

IPC分类号： C12P21/02 ,  C12N1/20 ,  C12N15/52 ,  C12N9/14 ,  C12P5/00 ,  C12P19/02

摘要： The present invention provides a method for producing a target substance, the biosynthetic pathway of which is ATP-dependent, such as, for example, amino acids, nucleosides, nucleotides, isoprenoids, and peptides, by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having H+-translocating membrane-bound pyrophosphatase activity, such as, for example, the hppA gene native to R. rubrum or a variant thereof.