公开(公告)号：US20220251622A1

公开(公告)日：2022-08-11

申请号：US17730750

申请日：2022-04-27

Applicant: AJINOMOTO CO., INC.

Inventor： Yasumichi Koseki ,  Takahiro Katayama ,  Yusuke Ooya ,  Fumi Ogata ,  Kiyoshi Hirakawa ,  Takuya Higuchi

IPC: C12P21/02 ,  C12N5/00

Abstract: A method for producing an objective protein using animal cells as an expression host is provided. The objective protein is produced by culturing animal cells having an objective protein-producing ability under conditions where the phosphoric acid concentration and/or the potassium concentration in the culture medium is increased.

公开(公告)号：US20250163487A1

公开(公告)日：2025-05-22

申请号：US18839852

申请日：2023-02-20

Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHA ,  AJINOMOTO CO., INC.

Inventor： Nobumasa Takao ,  Takuya Higuchi ,  Shumpei Furomitsu

IPC: C12P21/00 ,  C07K16/00 ,  C12N5/00

Abstract: Provided are a perfusion culture method and the like that are able to improve the productivity in the production of recombinant protein. Specifically, a method for improving recombinant protein production in perfusion culture of an animal cell having a capacity to produce a recombinant protein, the method comprising adding cystine and/or ammonium iron citrate to a cell culture medium, and the like, are provided.

公开(公告)号：US20240344021A1

公开(公告)日：2024-10-17

申请号：US18750252

申请日：2024-06-21

Applicant: AJINOMOTO CO., INC.

Inventor： Shinya Yamaide ,  Masashi Harada ,  Akari Tashiro ,  Chihiro Tsuji ,  Takuya Higuchi ,  Satoshi Okutani ,  Fumi Ogata ,  Shumpei Furomitsu ,  Masakazu Sugiyama

IPC: C12N5/00

CPC classification number: C12N5/0018 ,  C12N2500/32 ,  C12N2500/60

Abstract: A composition for animal cell culture and techniques relating thereto are provided. A composition for animal cell culture, which contains an amino acid source and α-ketoglutaric acid, and has the following properties: (A) the α-ketoglutaric acid is a salt of α-ketoglutaric acid; and/or (B) the amino acid source is free L-cysteine anhydrate is provided.