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公开(公告)号：US09157127B2
公开(公告)日：2015-10-13
申请号：US14183113
申请日：2014-02-18
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q1/689 , C12Q2600/158 , G01N21/6428 , G01N21/6456 , G01N2021/6432 , Y02A50/53 , C12Q2521/507 , C12Q2522/101 , C12Q2527/137 , C12Q2565/629 , C12Q2563/155 , C12Q2563/159
摘要： A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.
摘要翻译： 一种方法包括提供包含重组酶，单链结合蛋白和一种或多种寡核苷酸的混合物; 并检测反应混合物中的颗粒。
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公开(公告)号：US20200232050A1
公开(公告)日：2020-07-23
申请号：US16081941
申请日：2017-03-03
申请人： ALERE SAN DIEGO INC
发明人： Murray John Whyte , Niall A. Armes , Olaf Piepenburg , Catherine Jean Greenwood , Oliver Nentwich
IPC分类号： C12Q1/70 , C12Q1/686 , C12Q1/6806 , G01N27/416
摘要： A flu assay system including a sample module, a microfluidic nucleic acid amplification device, and an analyzer to facilitate fully automated nested recombinase polymerase amplification (RPA) on a sample delivered to the nucleic acid amplification device via the sample module. The assay includes providing a sample to a microfluidic device, and amplifying a target polynucleotide sequence in the sample. Amplifying the target polynucleotide sequence includes performing a first round of amplification on the sample to yield a first amplification product, and performing a second round of amplification on the first amplification product to yield a second amplification product. The second amplification product includes a smaller sequence completely contained within the first amplification product produced during the first round of amplification.
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公开(公告)号：US20160068898A1
公开(公告)日：2016-03-10
申请号：US14841203
申请日：2015-08-31
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q1/689 , C12Q2600/158 , G01N21/6428 , G01N21/6456 , G01N2021/6432 , Y02A50/53 , C12Q2521/507 , C12Q2522/101 , C12Q2527/137 , C12Q2565/629 , C12Q2563/155 , C12Q2563/159
摘要： A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.
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公开(公告)号：US20150024397A1
公开(公告)日：2015-01-22
申请号：US14183113
申请日：2014-02-18
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q1/689 , C12Q2600/158 , G01N21/6428 , G01N21/6456 , G01N2021/6432 , Y02A50/53 , C12Q2521/507 , C12Q2522/101 , C12Q2527/137 , C12Q2565/629 , C12Q2563/155 , C12Q2563/159
摘要： A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.
摘要翻译： 一种方法包括提供包含重组酶，单链结合蛋白和一种或多种寡核苷酸的混合物; 并检测反应混合物中的颗粒。
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公开(公告)号：US20190078147A1
公开(公告)日：2019-03-14
申请号：US16131580
申请日：2018-09-14
申请人： ALERE SAN DIEGO INC.
发明人： Michael L. Powell , Frank Ray Bowler , Catherine Jean Greenwood , Olaf Piepenburg , Niall A. Armes
IPC分类号： C12Q1/6818 , C12Q1/6844 , A61K31/713 , C12Q1/6888 , B01L3/00 , C12Q1/6881
摘要： This disclosure relates to methods and compositions for detecting a target nucleic acid sequence using a dual-hapten probe. More specifically, the present disclosure relates to using recombinase polymerase amplification (RPA) and a dual-hapten probe to detect a target nucleic acid sequence. In some cases, the detection is on lateral flow strips.
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公开(公告)号：US20180243739A1
公开(公告)日：2018-08-30
申请号：US15906899
申请日：2018-02-27
申请人： Alere San Diego Inc.
发明人： Alexander Schenk zu Schweinsberg , Austin Matthew Derfus , Justin Davidson , Karthikeyan Kumaravadivelu , Maulik Vinod Patel , Niall A. Armes , Olaf Piepenburg , Catherine Jean Greenwood , Oliver Nentwich
摘要： A microfluidic device includes an inlet port configured to receive a sample, a first reaction chamber fluidically coupled to the inlet port, a first pump fluidically coupled to the inlet port, a second pump fluidically coupled to a mixing chamber, a metering channel fluidically coupled to the first reaction chamber and to the mixing chamber, and one or more second reaction chambers fluidically coupled to the mixing chamber. The first pump is configured to move fluid from the inlet port to the first reaction chamber and from the first pump to the inlet port. The second pump is configured to move fluid from the second pump to the mixing chamber, from the first reaction chamber to the mixing chamber, and from the mixing chamber to the one or more second reaction chambers.
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公开(公告)号：US09719132B2
公开(公告)日：2017-08-01
申请号：US14841203
申请日：2015-08-31
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q1/689 , C12Q2600/158 , G01N21/6428 , G01N21/6456 , G01N2021/6432 , Y02A50/53 , C12Q2521/507 , C12Q2522/101 , C12Q2527/137 , C12Q2565/629 , C12Q2563/155 , C12Q2563/159
摘要： A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.
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