利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

39 条记录 (耗时 0.066 秒)

    Recombinase polymerase amplification
    1.
    发明授权
    Recombinase polymerase amplification 有权

    公开(公告)号：US10329603B2

    公开(公告)日：2019-06-25

    申请号：US15496756

    申请日：2017-04-25

    申请人： Alere San Diego Inc.

    发明人： Olaf Piepenburg ,  Colin H. Williams ,  Niall A. Armes ,  Derek L. Stemple

    IPC分类号： C12P19/34 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12N9/12 ,  B01L7/00 ,  C12Q1/6818 ,  G01N33/53

    摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.

    Recombinase Polymerase Amplification
    6.
    发明申请
    Recombinase Polymerase Amplification 审中-公开

    公开(公告)号：US20160083780A1

    公开(公告)日：2016-03-24

    申请号：US14865707

    申请日：2015-09-25

    申请人： Alere San Diego, Inc.

    发明人： Olaf Piepenburg ,  Colin H. Williams ,  Niall A. Armes ,  Derek L. Stemple

    IPC分类号： C12Q1/68 ,  B01L7/00 ,  G01N33/53

    CPC分类号： C12Q1/6844 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/18 ,  C12N9/1252 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Y207/07007 ,  G01N33/5308 ,  C12Q2531/113 ,  C12Q2522/101 ,  C12Q2521/507 ,  C12Q2527/125

    摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.

    Recombinase polymerase amplification
    7.
    发明授权
    Recombinase polymerase amplification 有权

    公开(公告)号：US10093908B2

    公开(公告)日：2018-10-09

    申请号：US14135227

    申请日：2013-12-19

    申请人： Alere San Diego, Inc.

    发明人： Olaf Piepenburg ,  Niall A. Armes ,  Mathew James David Parker

    IPC分类号： C07K14/00 ,  C12N9/12 ,  C12N9/00 ,  C12Q1/6844

    摘要： The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.

    DETECTION OF NUCLEIC ACIDS IN CRUDE MATRICES
    8.
    发明申请
    DETECTION OF NUCLEIC ACIDS IN CRUDE MATRICES 审中-公开

    公开(公告)号：US20170335379A1

    公开(公告)日：2017-11-23

    申请号：US15612418

    申请日：2017-06-02

    申请人： Alere San Diego Inc.

    发明人： Niall A. Armes

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6846 ,  Y02A50/53 ,  C12Q2527/101

    摘要： A method includes contacting a crude matrix with components of an isothermal nucleic acid amplification reaction for a target nucleic acid species, thereby providing a mixture; incubating the mixture under conditions sufficient for the isothermal nucleic acid amplification reaction to proceed, thereby providing a product; and determining whether an indicator of the target nucleic acid species is present in the product.

    Recombinase Polymerase Amplification
    10.
    发明申请
    Recombinase Polymerase Amplification 审中-公开
    重组酶聚合酶扩增

    公开(公告)号：US20160326502A1

    公开(公告)日：2016-11-10

    申请号：US14135227

    申请日：2013-12-19

    申请人： Alere San Diego, Inc.

    发明人： Olaf Piepenburg ,  Niall A. Armes ,  Matthew James David Parker

    IPC分类号： C12N9/12 ,  C12Q1/68

    CPC分类号： C12N9/1241 ,  C12N9/00 ,  C12Q1/6844 ,  C12Y207/07 ,  C12Q2521/507 ,  C12Q2531/119

    摘要： The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.

    摘要翻译： 本发明的特征在于新颖，多样，杂交和工程重组酶，以及具有相关重组因子的这种蛋白质用于进行DNA扩增测定。 本发明还具有在DNA扩增测定中具有不同生物化学活性的不同重组酶“系统”，对负载因子，单链DNA结合蛋白（SSB）以及所用的拥挤剂的量的不同要求。