公开(公告)号：US20230048658A1

公开(公告)日：2023-02-16

申请号：US17825793

申请日：2022-05-26

Applicant: AMGEN INC.

Inventor： Trent P. MUNRO ,  Neeraj Jagdish AGRAWAL ,  Kristine M. DARIS

IPC: C07K16/18 ,  C12N15/65 ,  C12N15/67 ,  C07K16/06 ,  C12N15/85

Abstract: This invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More specifically, the invention concerns improved selection of cells transfected with recombinantly engineered vectors designed to express polypeptides, in particular heteromultimeric polypeptides.

公开(公告)号：US20190127452A1

公开(公告)日：2019-05-02

申请号：US16099986

申请日：2017-05-11

Applicant: AMGEN INC.

Inventor： Randal Robert KETCHEM ,  Jeffrey T. MCGREW ,  Dina A. FOMINA YADLIN ,  Trent P. MUNRO ,  Neeraj Jagdish AGRAWAL ,  Kristine M. DARIS

IPC: C07K16/18 ,  C12N15/67 ,  C12N15/65 ,  C12N15/85 ,  C07K16/06

Abstract: This invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More specifically, the invention concerns improved selection of cells transfected with recombinantly engineered vectors designed to express polypeptides, in particular heteromultimeric polypeptides.

公开(公告)号：US20220064610A1

公开(公告)日：2022-03-03

申请号：US17299000

申请日：2019-12-09

Applicant: AMGEN INC.

Inventor： Neeraj J. AGRAWAL ,  Kristine M. DARIS ,  Jennitte L. STEVENS ,  Huong Thi Ngoc LE ,  Noelia Blanco TALAVAN

IPC: C12N9/12 ,  C12N15/85 ,  C07K14/725 ,  C12N15/90 ,  A61K35/17

Abstract: PiggyBac transposases engineered to increase stability in a cell. The engineered piggyBac transposases are useful for stably transforming cells, cell line development, genome modification, and improving titer of recombinant proteins, among other uses.