公开(公告)号：US11192919B2

公开(公告)日：2021-12-07

申请号：US16042965

申请日：2018-07-23

Applicant: AMGEN INC.

Inventor： Samuel Ray Trejo ,  Robert Perry Brake

IPC: C07K1/18 ,  B01D15/38 ,  C07K16/00 ,  B01D15/36 ,  C07K14/705 ,  C07K14/715 ,  B01D15/18 ,  C07K1/22

Abstract: The invention provides for the removal of a large fraction of contaminants from protein preparations while maintaining a high level of recovery using tentacle anion exchange matrix chromatography medium. Using the methods of the invention, leached affinity chromatography contaminants can be removed from recombinant protein preparations.

公开(公告)号：US20220396599A1

公开(公告)日：2022-12-15

申请号：US17775386

申请日：2020-11-13

Applicant: AMGEN INC.

Inventor： Yan Wang ,  Nicholas Marchand ,  Robert Perry Brake ,  Maria Perry ,  Ashish Sharma ,  Eugene Sun

IPC: C07K1/34 ,  C07K16/28

Abstract: The present disclosure provides a downstream manufacturing method for the production of bispecific antigen-binding molecule products, which comprise at least two different binding domains. The process comprises at least one filtration step, wherein the filtration is ultrafiltration/diafiltration (UF/DF), and/or viral filtration (VF), wherein a β-cyclodextrin is applied in the process either in a buffer applied in the filtration step or added to the filtration pool after the UF/DF filtration step wherein the buffer or the pool have a concentration of about 0.1 to 3% (m/v) of a (3-cyclodextrin, to preferably keep the product in a non-aggregated form.

公开(公告)号：US11492372B2

公开(公告)日：2022-11-08

申请号：US17554865

申请日：2021-12-17

Applicant: AMGEN INC.

Inventor： Samuel Ray Trejo ,  Robert Perry Brake

IPC: B01D15/36 ,  B01D15/38 ,  C07K1/18 ,  C07K16/00 ,  C07K1/22 ,  C07K14/705 ,  C07K14/715 ,  B01D15/18

Abstract: The invention provides for the removal of a large fraction of contaminants from protein preparations while maintaining a high level of recovery using tentacle anion exchange matrix chromatography medium. Using the methods of the invention, leached affinity chromatography contaminants can be removed from recombinant protein preparations.