公开(公告)号：US20240327875A1

公开(公告)日：2024-10-03

申请号：US18578609

申请日：2022-07-15

Applicant: AMYRIS, INC.

Inventor： Quinn Mitrovich ,  William E. Draper ,  Andrew Klein ,  Michelle Medina

IPC: C12P7/04 ,  C12N1/16 ,  C12N9/16 ,  C12N15/81 ,  C12R1/865

CPC classification number: C12P7/04 ,  C12N1/165 ,  C12N9/16 ,  C12N15/81 ,  C12N2510/00 ,  C12R2001/865 ,  C12Y306/01

Abstract: The present disclosure features compositions and methods for producing one or more isoprenoid compounds, such as E-copalol, in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of an isoprenoid biosynthetic pathway, such as a pathway for making E-copalol. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of an isoprenoid biosynthetic pathway, such as a copalyl-diphosphate (CPP) pyrophosphatase. The host cell may then be cultured in a medium, for example, in the presence of an agent that regulates expression of the one or more enzymes. The host cell may further be incubated for a time sufficient to allow for production of an isoprenoid compound, such as E-copalol, by the host cell. The isoprenoid compound may then be separated from the host cell or from the medium.