公开(公告)号：US20240327875A1

公开(公告)日：2024-10-03

申请号：US18578609

申请日：2022-07-15

Applicant: AMYRIS, INC.

Inventor： Quinn Mitrovich ,  William E. Draper ,  Andrew Klein ,  Michelle Medina

IPC: C12P7/04 ,  C12N1/16 ,  C12N9/16 ,  C12N15/81 ,  C12R1/865

CPC classification number: C12P7/04 ,  C12N1/165 ,  C12N9/16 ,  C12N15/81 ,  C12N2510/00 ,  C12R2001/865 ,  C12Y306/01

Abstract: The present disclosure features compositions and methods for producing one or more isoprenoid compounds, such as E-copalol, in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of an isoprenoid biosynthetic pathway, such as a pathway for making E-copalol. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of an isoprenoid biosynthetic pathway, such as a copalyl-diphosphate (CPP) pyrophosphatase. The host cell may then be cultured in a medium, for example, in the presence of an agent that regulates expression of the one or more enzymes. The host cell may further be incubated for a time sufficient to allow for production of an isoprenoid compound, such as E-copalol, by the host cell. The isoprenoid compound may then be separated from the host cell or from the medium.

公开(公告)号：US20250002953A1

公开(公告)日：2025-01-02

申请号：US18578903

申请日：2022-07-11

Applicant: Amyris, Inc.

Inventor： William E. Draper ,  John E. Hung ,  Alex McGill ,  Victor Holmes

IPC: C12P17/06 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12P7/42

Abstract: The present disclosure features compositions and methods for producing one or more cannabinoids, such as cannabidiolic acid (CBDa) or cannabidiol (CBD), in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of a cannabinoid biosynthetic pathway. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as CBDaS. The host cell may be cultured in the medium in the presence of a first cannabinoid, for example CBGa, and incubated for a time sufficient to allow for bioconversion of the first cannabinoid to a second cannabinoid, for example CBDa, by the host cell.

公开(公告)号：US20240344093A1

公开(公告)日：2024-10-17

申请号：US18578649

申请日：2022-07-11

Applicant: AMYRIS, INC.

Inventor： John E. Hung ,  William E. Draper ,  Victor Holmes

IPC: C12P7/42 ,  C12N9/02 ,  C12N15/62 ,  C12N15/81 ,  C12P7/22

CPC classification number: C12P7/42 ,  C12N9/0004 ,  C12N15/62 ,  C12N15/81 ,  C12P7/22 ,  C07K2319/02 ,  C07K2319/50 ,  C12Y121/03008

Abstract: The present disclosure features compositions and methods for producing one or more cannabinoids, such as cannabidiolic acid (CBDa), in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of a cannabinoid biosynthetic pathway. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of a cannabinoid biosynthetic pathway, such as an enzyme having CBDa synthase (CBDaS) activity.

公开(公告)号：US20240327881A1

公开(公告)日：2024-10-03

申请号：US18578894

申请日：2022-07-15

Applicant: Amyris, Inc.

Inventor： Quinn Mitrovich ,  William E. Draper ,  Michelle Medina

IPC: C12P7/62 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/14

CPC classification number: C12P7/62 ,  C12N9/0006 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/14 ,  C12Y101/01001 ,  C12Y205/01029 ,  C12Y306/01

Abstract: The present disclosure features compositions and methods for producing one or more isoprenoid compounds, such as gamma-ambryl acetate (GAA), in a host cell, such as a yeast cell, that is genetically modified to express the enzymes of an isoprenoid biosynthetic pathway, such as a pathway for making GAA. Using the compositions and methods of the present invention, the host cell may be genetically modified to express one or more enzymes of an isoprenoid biosynthetic pathway, such as an enzyme capable of converting manooloxy to GAA. The host cell may be cultured in a medium, for example, in the presence of an agent that regulates expression of the one or more enzymes. The host cell may be incubated for a time sufficient to allow for production of an isoprenoid compound, such as GAA. The isoprenoid compound may then be separated from the host cell or from the medium.