公开(公告)号：US11085046B2

公开(公告)日：2021-08-10

申请号：US15771888

申请日：2016-10-28

Applicant: AMYRIS, INC.

Inventor： Andrew Main ,  Grzegorz Wojciechowski ,  Yue Yang ,  Lishan Zhao

IPC: C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N15/52 ,  C12P5/00

Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyrophosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.

公开(公告)号：US11767533B2

公开(公告)日：2023-09-26

申请号：US17371678

申请日：2021-07-09

Applicant: AMYRIS, INC.

Inventor： Andrew Main ,  Grzegorz Wojciechowski ,  Yue Yang ,  Lishan Zhao

IPC: C12N1/16 ,  C12N15/52 ,  C12P5/00 ,  C12N1/20 ,  C12N1/14

CPC classification number: C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P5/007 ,  C12Y205/01029 ,  C12Y402/03015

Abstract: Provided herein are compositions and methods for producing myrcene by culturing genetically modified microbial host cells that express a myrcene synthase and optionally a geranyl pyroplosphate synthase. Also provided herein are isolated nucleic acid molecules that encode myrcene synthase variants derived from the Ocimum species myrcene synthase, which comprise one or more amino acid substitutions that improve in vivo performance of myrcene synthase in genetically modified microbial host cells. Also provided herein are isolated myrcene synthase variants that exhibit an improved activity for converting geranyl diphosphate into myrcene.