公开(公告)号：US20210403983A1

公开(公告)日：2021-12-30

申请号：US17370859

申请日：2021-07-08

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shengxi Chen ,  Sidney Hecht ,  Mingxuan Gao

IPC: C12Q1/6806 ,  C12Q1/70 ,  C12Q1/686 ,  C12Q1/6818 ,  C12Q1/682

Abstract: Described herein are compositions that may be used to detect viral nucleic acid. For example, these compositions may comprise a DNA-nanostructure, a capture oligonucleotide and a protector oligonucleotide, wherein the components are designed based on a duo-toehold-mediated displacement reaction (duo-TMDR) strategy. In this strategy, a first TMDR can switch off a Faster resonance energy transfer (FRET) process and a second TMDR can release the target viral nucleic acid and amplify the signal. Methods of using such compositions are also provided herein.

公开(公告)号：US20180371526A1

公开(公告)日：2018-12-27

申请号：US16016086

申请日：2018-06-22

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shengxi Chen ,  Sidney Hecht ,  Mingxuan Gao

IPC: C12Q1/6806 ,  C12Q1/686 ,  C12Q1/70

Abstract: Described herein are compositions that may be used to detect viral nucleic acid. For example, these compositions may comprise a DNA-nanostructure, a capture oligonucleotide and a protector oligonucleotide, wherein the components are designed based on a duo-toehold-mediated displacement reaction (duo-TMDR) strategy. In this strategy, a first TMDR can switch off a Foster resonance energy transfer (FRET) process and a second TMDR can release the target viral nucleic acid and amplify the signal. Methods of using such compositions are also provided herein.