公开(公告)号：US10246726B2

公开(公告)日：2019-04-02

申请号：US15938742

申请日：2018-03-28

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Bo Wang ,  Weiwen Zhang ,  Deirdre Meldrum ,  David Nielsen

IPC: C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12P7/52 ,  C07C59/01

Abstract: Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.

公开(公告)号：US20180312884A1

公开(公告)日：2018-11-01

申请号：US15938742

申请日：2018-03-28

Applicant: Arizona Board Of Regents On Behalf Of Arizona State University

Inventor： Bo Wang ,  Weiwen Zhang ,  Deirdre Meldrum ,  David Nielsen

IPC: C12P7/52 ,  C07C59/01 ,  C12N9/04 ,  C12N9/16 ,  C12N9/10

CPC classification number: C12P7/52 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  C12Y101/01036 ,  C12Y101/01157 ,  C12Y203/01009 ,  C12Y301/0202 ,  Y02P20/134

Abstract: Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.