公开(公告)号：US11708590B2

公开(公告)日：2023-07-25

申请号：US16631232

申请日：2018-07-16

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： David Nielsen ,  Brian Thompson

IPC: C12P7/18 ,  C12P7/46 ,  C12N9/02 ,  C12N9/88 ,  C12N9/90 ,  C12P7/22

CPC classification number: C12P7/46 ,  C12N9/0069 ,  C12N9/0073 ,  C12N9/88 ,  C12N9/90 ,  C12P7/22 ,  C12Y113/11001 ,  C12Y114/13002 ,  C12Y114/13007 ,  C12Y401/01061 ,  C12Y401/01063 ,  C12Y401/01091 ,  C12Y401/0304 ,  C12Y401/99002 ,  C12Y402/0101 ,  C12Y402/99021 ,  C12Y504/04002

Abstract: Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.

公开(公告)号：US20230203542A1

公开(公告)日：2023-06-29

申请号：US18111101

申请日：2023-02-17

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： David Nielsen ,  Michael Machas ,  Rebekah McKenna

IPC: C12P7/22 ,  C12N9/88 ,  C12N1/20 ,  C12R1/19

CPC classification number: C12P7/22 ,  C12N1/20 ,  C12N9/88 ,  C12N1/205 ,  C12R2001/19 ,  C12Y102/01039 ,  C12Y114/14011 ,  C12Y403/01024 ,  C12Y503/99007

Abstract: Described herein are engineered metabolic pathways in recombinant microorganism host cells which result in the production of 2-phenylethanol or 2-phenylacetic acid. Also described herein are methods of using the recombinant microorganisms for the production of 2-phenylethanol or 2-phenylacetic acid.

公开(公告)号：US11613768B2

公开(公告)日：2023-03-28

申请号：US16633525

申请日：2018-07-18

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： David Nielsen ,  Michael Machas ,  Rebekah McKenna

IPC: C12N1/20 ,  C12P7/22 ,  C12N9/88 ,  C12R1/19

Abstract: Described herein are engineered metabolic pathways in recombinant microorganism host cells which result in the production of 2-phenylethanol or 2-phenylacetic acid. Also described herein are methods of using the recombinant microorganisms for the production of 2-phenylethanol or 2-phenylacetic acid.

公开(公告)号：US20190194695A1

公开(公告)日：2019-06-27

申请号：US16184377

申请日：2018-11-08

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： David Nielsen ,  Shawn Pugh ,  Rebekah McKenna

IPC: C12P5/02 ,  C12N9/88

CPC classification number: C12P5/026 ,  C12N9/88 ,  C12Y401/01 ,  Y02E50/343

Abstract: A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.

公开(公告)号：US20200231992A1

公开(公告)日：2020-07-23

申请号：US16633525

申请日：2018-07-18

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： David Nielsen ,  Michael Machas ,  Rebekah McKenna

IPC: C12P7/22 ,  C12N9/88 ,  C12N1/20

Abstract: Described herein are engineered metabolic pathways in recombinant microorganism host cells which result in the production of 2-phenylethanol or 2-phenylacetic acid. Also described herein are methods of using the recombinant microorganisms for the production of 2-phenylethanol or 2-phenylacetic acid.

公开(公告)号：US10246726B2

公开(公告)日：2019-04-02

申请号：US15938742

申请日：2018-03-28

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Bo Wang ,  Weiwen Zhang ,  Deirdre Meldrum ,  David Nielsen

IPC: C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12P7/52 ,  C07C59/01

Abstract: Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.

公开(公告)号：US20180312884A1

公开(公告)日：2018-11-01

申请号：US15938742

申请日：2018-03-28

Applicant: Arizona Board Of Regents On Behalf Of Arizona State University

Inventor： Bo Wang ,  Weiwen Zhang ,  Deirdre Meldrum ,  David Nielsen

IPC: C12P7/52 ,  C07C59/01 ,  C12N9/04 ,  C12N9/16 ,  C12N9/10

CPC classification number: C12P7/52 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  C12Y101/01036 ,  C12Y101/01157 ,  C12Y203/01009 ,  C12Y301/0202 ,  Y02P20/134

Abstract: Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.